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从传统发酵大豆奶酪中鉴定和表征乌玛米-ACE抑制
Guanmian Wei1, Feiran Zhao2, Ziyi Zhang2
1College of Food Science and Technology, Hebei Agricultural University, Baoding, Hebei Province 071001, PR China; School of Food Science, Jiangnan University, Wuxi, Jiangsu Province 214122, PR China.
Food chemistry
|November 23, 2024
概括
研究人员从发酵大豆 (FSC) 中鉴定出强大的乌玛米和血管酶转化酶 (ACE) 抑制. 这些,像WEEF一样,显示出作为功能性食品成分的承诺,用于增强味道和心血管健康.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 发酵大豆奶酪 (FSC) 由于其乌玛米味道和潜在的生物活性而受到重视.
- 在食品来源中识别生物活性对于开发功能性食品至关重要.
研究的目的:
- 为了识别和表征FSC的,具有乌玛米味道和血管酶转化酶 (ACE) 抑制活性.
- 评估这些作为功能成分的潜力.
主要方法:
- 纳米-HPLC-MS/MS用于识别FSC中的.
- 生物信息查和药模拟用于预测ACE抑制潜力.
- 合成了三种高活性 (VE,FEF,WEEF),并确定了它们的乌玛米值和抑制ACE的IC50值.
- 进行了氧化 (NO) 和内甲素-1 (ET-1) 生产试验.
主要成果:
- 确定了11种具有潜在乌玛米和ACE抑制活性的候选.
- 药模拟表明高ACE抑制概率,主要通过键,静电和疏水相互作用结合.
- 合成的类VE,FEF和WEEF表现出不同的umami值和ACE抑制功效,WEEF显示出最强的ACE抑制 (85 ± 2μM IC50).
- 抑制ACE的活性是剂量依赖的,影响NO和ET-1的产生.
结论:
- 来自发酵大豆的特定具有显著的乌玛米味道和 ACE 抑制性质.
- 织物,FEF和VE被确定为功能性食品应用的有希望的候选者,可能有助于心血管健康.
- 这项研究提出了一种可行的选方法,用于选具有Umami和ACE抑制活性的胺.
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