相关实验视频

Updated: Jun 6, 2025

Improved Visualization of Lung Metastases at Single Cell Resolution in Mice by Combined In-situ Perfusion of Lung Tissue and X-Gal Staining of lacZ-Tagged Tumor Cells

Improved Visualization of Lung Metastases at Single Cell Resolution in Mice by Combined In-situ Perfusion of Lung Tissue and X-Gal Staining of lacZ-Tagged Tumor Cells

Published on: August 21, 2012

艾兰能向KMT2A-MEN1复合体,以抑制骨髓瘤的肺转移

Jinrong Liang¹, Guanglei Qiao², Yawen Zhang³

¹Department of Oncology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200336, China; Department of Oncology, Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200233, China.

Phytomedicine : international journal of phytotherapy and phytopharmacology

|November 23, 2024

概括

艾兰 (AIL) 向KMT2A-MEN1复合体,抑制血清蛋白生物合成以抑制骨肉瘤肺转移. 这种天然产品提供了一种新的治疗策略,与目前的治疗方法不同.

科学领域:

在瘤学瘤学.
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
药理学药理学是指药理学的学科.

背景情况:

肺转移是骨髓瘤 (OS) 中的主要死亡原因.
向表观遗传重编程为OS肺转移提供了一个新的治疗途径.
天然产品艾兰 (Ailanthone,AIL) 抑制OS生长,但其直接目标尚不清楚.

研究的目的:

在OS中确定AIL的直接目标.
在体内研究AIL对OS肺转移的作用.

主要方法:

表面等离子体共振-高性能液体染色学-质谱学 (SPR-HPLC-MS) 以确定AIL目标.
在体外测试 (热转移,对接,酶活性,qRT-PCR,西斑,ChIP) 以确认目标和途径.
在体内小鼠异种移植模型来评估AIL的疗效和机制.

主要成果:

艾尔直接针对希斯-氨酸N-甲基转移酶2A (KMT2A) 和它的支架蛋白menin (MEN1).
AIL诱导KMT2A-MEN1复合物的自降解,抑制H3K4甲基转移酶的活性.
AIL在表观遗传上抑制了血清蛋白生物合成途径 (SSP),并在体内减少OS肺转移.

结论:

艾尔针对KMT2A-MEN1复合体并抑制SSP,从而抑制OS肺转移.
AIL展示了与现有的OS疗法不同的新型作用机制.
使用AIL向表观遗传酶和癌症代谢,为OS提供了一个有前途的治疗策略.

关键词:

艾兰托尼 (Ailanthone) 是一种音调.在 KMT2A 中.男人,就是一个人.转移转移转移转移骨质肉瘤骨质肉瘤是什么 PHGDH PHGDH 的时间

更多相关视频

Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos

Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos

Published on: December 13, 2018

Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer

Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer

Published on: July 21, 2018

相关概念视频

Targeted Cancer Therapies

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...