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在体外成熟过程中补充L-kynurenine可以通过其抗氧化作用改善牛卵细胞的发育能力

Canqiang Lu¹, Yun Feng², Zhenhua Tang³

¹State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Guangxi University, Nanning, 530005, China.

|November 23, 2024

概括

补充L-kynurenine (L-KYN) 显著改善了牛卵细胞成熟和胚胎发育. 这种抗氧化剂通过减少体外成熟过程中的氧化应激和亡来提高卵细胞质量.

科学领域:

生殖生物学生殖生物学
生物化学生化学
细胞生物学细胞生物学

背景情况:

氧化应激在体外成熟 (IVM) 过程中对卵细胞发育潜力产生负面影响.
L-kynurenine (L-KYN) 是一种内源代谢物,具有抗氧化,抗炎和神经保护性质.
确定有效的补充剂来改善IVM中的卵细胞质量对于辅助生殖技术至关重要.

研究的目的:

研究L-kynurenine (L-KYN) 对牛卵细胞IVM的影响.
阐明L-KYN在卵细胞成熟过程中的作用的基本机制.
评估L-KYN作为一种潜在的抗氧化剂补充剂,用于改善牛卵细胞质量.

主要方法:

牛卵细胞在体外成熟时具有不同度的L-KYN (0,10,50,100,200μmol/L).
评估了卵细胞成熟率,胚胎分裂率和胚胎细胞形成率.
评估了基因表达分析 (Bax,Caspase3,HAS2,PTX3,PTGS2,GPX4,CAT),谷氨 (GSH) 水平,线粒体活性,DNA修复,活性氧物种 (ROS) 和亡.

主要成果:

100μmol/L L-KYN显著增强了卵细胞成熟,胚胎裂变和胚胎细胞形成率.
L-KYN治疗降低了与亡相关的基因 (Bax,Caspase3) 的调节,并提高了累积扩张和抗氧化基因 (HAS2,PTX3,PTGS2,GPX4,CAT) 的调节.
L-KYN增加了GSH水平,线粒体活性和DNA修复,同时降低了ROS和亡.

结论:

用100μmol/L的L-KYN补充剂改善了IVM期间的牛卵细胞质量和随后的胚胎发育.
L-KYN 作为抗氧化剂,减轻卵细胞中的氧化应激和亡.
L-KYN是一种有前途的抗氧化剂补充剂,可在体外优化牛卵细胞成熟.

关键词:

抗氧化剂是一种抗氧化剂.牛类牛类牛类牛类牛类牛在体外成熟.这是一种L-kynurenine.卵子细胞是卵子细胞.

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