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Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

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Published on: June 16, 2015

提高细胞外CaCl的效果:对于组织工程应用的重要考虑

Kayley Jaworska¹, Jessica J Senior¹, Anke Brüning-Richardson²

¹Department of Pharmacy, School of Applied Sciences, University of Huddersfield, Queensgate, Huddersfield HD1 3DH, United Kingdom.

|November 23, 2024

概括

在水凝中化 (CaCl2) 度升高会对细胞活力,增殖,形态和组织工程中的迁移产生负面影响. 精心优化对于生物材料的适用性和标准化至关重要.

关键词:

生物相容性生物相容性生物材料是一种生物材料.是一种交叉连接 (crosslinking) 是一种交叉连接.质母细胞瘤 (glioblastoma) 是一个水凝是一种水凝.组织工程是组织工程.

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科学领域:

生物材料科学生物材料科学
组织工程是组织工程.
细胞生物学细胞生物学

背景情况:

聚糖类如酸盐,点和凝糖通过双价金属离子形成水凝,通常是化 (CaCl2).
这些水凝在组织工程中使用,但CaCl2升高对细胞行为的影响还未得到充分研究.
在CaCl2使用中缺乏标准化可能会影响生物材料的适用性和实验结果.

研究的目的:

研究细胞外CaCl2增加对细胞活力,增殖,形态和迁移的影响.
在使用质母细胞瘤,纤维母细胞和骨母细胞细胞系的组织工程模型中评估CaCl2使用的后果.
重新评估生物材料的适用性,并加强组织工程中的标准化实践.

主要方法:

使用了质母细胞瘤 (U251),人类皮肤纤维细胞 (HDF) 和小鼠骨质母细胞 (MC3T3) 细胞系.
在3D水凝中培养的细胞具有不同度的细胞外CaCl2.
分析了细胞活力,增殖,形态 (表型变化) 和迁移.

主要成果:

在质母细胞瘤细胞中,CaCl2诱导了显著的形态变化,在8毫米的时间内从介质细胞转变为阿米类表型.
在所有测试的细胞类型中,细胞活力以度依赖的方式下降.
细胞增殖和迁移也受到CaCl2度升高的负面影响.

结论:

高度的CaCl2,在促进水凝凝的同时,对细胞活力,增殖,形态和迁移构成重大风险.
在使用高度的CaCl2在组织工程中之前,细胞系特定的实验和标准化是必不可少的.
优化CaCl2度对于水凝在组织工程应用中准确地回顾细胞反应至关重要.