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产前尼松暴露的剂量,阶段和性别差异对胎盘形态和功能发育的影响
Xiaomeng Zha1, Man Fang1, Wen Zhong1
1Department of Obstetrics and Gynaecology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.
在怀孕期间使用普雷尼松改变了小鼠的胎盘形态和营养物质运输,特别是在雄性动物中,但没有影响胎儿的结果. 需要进一步的研究来确定人类的相关性.
科学领域:
- 生殖生物学 生殖生物学
- 发育毒理学 发展毒理学
- 药理学 药理学是指药理学的学科.
背景情况:
- 普雷尼松 (PPE) 是怀孕期间自身免疫性疾病的常见治疗方法.
- 人们对与使用PPE相关的潜在不良妊娠结果存在担忧.
研究的目的:
- 在小鼠模型中研究普得尼松对胎盘和胎儿发育的影响.
- 为了确定个人防护设备对怀孕结果的剂量和阶段依赖的影响.
主要方法:
- 已确立的普得尼松诱导的怀孕小鼠模型在不同的剂量 (0.25-1.0 mg/kg·d) 和妊娠阶段.
- 评估了胎盘形态,胎儿参数,细胞增殖,细胞亡和基因表达.
- 利用西方斑点和mRNA分析来评估分子途径,包括IGF2/IGF1R信号传递.
主要成果:
- 高剂量的PPE (1.0 mg/kg) 显著降低了胎盘体重,并改变了结点/迷宫区域的比率.
- 细胞增殖标志物下降,而胎盘中在某些剂量下亡增加.
- 营养物质运输基因表达被改变,在高剂量和整个怀孕期间,男性胎盘的显着变化.
- PPE抑制了IGF2/IGF1R信号通路,与细胞增殖和营养物质运输的变化相关.
结论:
- 普雷尼松酸可以改变胎盘形态和功能,影响营养物质的运输.
- 影响因PPE剂量,治疗阶段和胎儿性别而异.
- IGF2/IGF1R通路与PPE对胎盘的影响有关.
- 虽然小鼠研究结果具有信息意义,但由于不一致的增殖数据和无法解释的性别差异,需要进一步研究以确定对人类怀孕的相关性.
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