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博姆比克斯·莫里 (Bombyx mori) PAT4基因通过自性抑制了BmNPV感染和复制

Kaifang Jia¹, Jinyang Wang¹, Dan Jiang¹

¹Jiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, China; Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture and Rural Affairs, Sericultural Scientific Research Center, Chinese Academy of Agricultural Sciences, Zhenjiang 212100, China.

Journal of invertebrate pathology

|November 23, 2024

概括

丝虫中的质子辅助氨基酸载体4 (PAT4) 抑制了Bombyx mori核多重体病毒 (BmNPV) 的复制. 这种载体激活BmTORC1,抑制自并限制病毒扩散的资源.

科学领域:

分子生物学分子生物学
病毒学病毒学
昆虫科学昆虫科学昆虫科学

背景情况:

质子辅助氨基酸载体4 (PAT4) 是溶解物载体 (SLC) 36家族的一部分,参与氨基酸运输.
在本研究之前,PAT4在Bombyx mori (丝虫) 中的特定作用尚不清楚.

研究的目的:

为了克隆和描述Bombyx mori PAT4 (BmPAT4) 基因.
为了研究BmPAT4在对Bombyx mori核多重体病毒 (BmNPV) 感染的反应中的功能.
阐明BmPAT4影响病毒复制和细胞通路的机制.

主要方法:

聚合酶链反应 (PCR) 用于BmPAT4基因克隆和鉴定.
进行了生物信息分析以确定序列特征,包括跨膜域.
在不同的组织和病毒感染下分析了BmPAT4表达水平.
在BmN细胞中使用了基因沉默 (RNA干扰) 和过度表达技术.
使用定量方法评估了自途径基因表达.

主要成果:

克隆了BmPAT4,揭示了1,395 bp的开放读取框架,编码了464个氨基酸,有10个跨膜域.
BmPAT4与相关的昆虫物种具有很高的序列相似性,并且定位在细胞膜中,在所有丝虫组织中表达.
BmNPV感染显著提高了中肠,血淋巴和脂肪体中的BmPAT4表达.
过度表达BmPAT4抑制了BmNPV的增殖和降低了自基因表达,而RNA干扰促进了病毒复制和增加了自基因表达.
BmPAT4激活了BmTORC1,后者随后抑制了自,从而限制了BmNPV的资源.

结论:

BmPAT4在丝虫对抗BmNPV的抗病毒防御中起着至关重要的作用.
抗病毒机制涉及BmPAT4介导的BmTORC1激活,导致自抑制.
这项研究提供了BmPAT4在丝虫中的抗病毒活性的第一个证据,为宿主-病原体相互作用提供了洞察力.

关键词:

这是一种抗病毒药物.在BmNPVV中.在BmTORC1中,波姆比克斯 (Bombyx mori mori mori) 是一个有趣的动物.细胞自细胞自.在 PAT4 PAT4 中.

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