与利多卡因相比,暴露于articaine的施万细胞显示出不同的毒性途径
Gustavo H Rodrigues da Silva1, Luís F Mendes2, Gabriela Geronimo3
1CICECO-Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, Aveiro, Portugal; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas-SP, Brazil; Brazilian Biosciences National Laboratory, Brazilian Center for Research in Energy and Materials, Campinas-SP, Brazil.
阿尔蒂卡因 (ATC) 比利多卡因 (LDC) 在施万细胞中引起更显著的代谢变化和内质网膜应激. 这可能解释了ATC.
科学领域:
- 神经科学是一个神经科学.
- 生物化学 生物化学
- 毒理学 毒理学 毒理学
背景情况:
- 阿尔蒂卡因 (ATC) 是牙科中广泛使用的局部麻醉剂,以其有效性而闻名.
- 与其他局部麻醉剂相比,ATC报告的麻醉发生率更高.
- 导致ATC引起的麻醉的确切机制尚未完全理解.
研究的目的:
- 研究阿尔蒂卡因 (ATC) 对施万细胞的代谢和细胞效应.
- 为了比较ATC与利多卡因 (LDC) 的影响,这是一种标准的牙科局部麻醉剂.
- 阐明ATC与麻醉相关的潜在机制.
主要方法:
- 在施万细胞上进行了细胞外代谢流量分析.
- 基于NMR的代谢学被用来分析细胞代谢物.
- 用蛋白质表达分析来检测内质网膜应激和亡标记物.
主要成果:
- 与LDC相比,ATC显著增加了施万细胞的氧气消耗.
- 代谢学揭示了不同的代谢特征,其中ATC提高了细胞内氨基酸 (白氨酸,异氨酸,氨酸,氨酸,氨酸,氨酸,氨酸,氨酸,氨酸,氨酸,氨酸,氨酸,氨酸).
- 接受ATC治疗的细胞表现出增加的内质网膜应激和亡.
结论:
- 与利多卡因相比,阿尔蒂卡因在施万细胞中诱导了更深刻的代谢干扰和细胞应激.
- 在ATC治疗的细胞中,氨基酸水平升高和ER压力/亡的迹象可能会导致其神经毒性.
- 这些发现提供了关于局部麻醉剂的差异性毒性和与articaine相关的麻醉症的更高发病率的见解.
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