相关实验视频

Updated: Jun 6, 2025

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Published on: May 2, 2018

一项关于微生物根运河微生物群的实体采样方法的方法学研究,用于下一代基因测序分析

David Donnermeyer¹, Johannes Matern², Karola Prior²

¹Department of Periodontology and Operative Dentistry, University of Münster, Münster, Germany; Department of Restorative, Preventive and Pediatric Dentistry, School of Dental Medicine, University of Bern, Bern, Switzerland.

Journal of endodontics

|November 23, 2024

概括

纸张点和内牙科文件都有效地为16S核糖体DNA (rDNA) 测序采样口腔微生物群. 建议使用内脏牙档案,以便在临床环境中更容易执行.

科学领域:

微生物学微生物学
基因组学就是基因组学.
牙周内科医院牙周内科医院牙周内科医院

背景情况:

研究体内内牙微生物群对于理解顶端牙周炎至关重要.
目前的采样方法需要对其在微生物研究中的适用性进行评估.

研究的目的:

为了比较纸质点的有效性与内牙科内牙科检查中的微生物采样的尼克尔-文件.
评估这些方法对于16S核糖体DNA (rDNA) 测序的适用性.

主要方法:

微生物样本从45名患有角性牙周炎的患者的84个根管中采集了两份,使用无菌纸点和无菌内牙科文件.
提取了DNA,并使用Illumina MiSeq.放大了16SrRNA基因的V4区域并进行了测序.
用生物信息工具和统计测试分析了微生物社区组成和多样性的测序数据.

主要成果:

阿尔法多样性在丰富性和不相似性上显示出非显著的差异,但在纸质点和文件样本之间的香农指数中显示出显著的差异.
贝塔多样性分析显示,两种采样方法之间的微生物群体组成相似.
纸质点的控制显示Pseudomonadales的比例明显更高.

结论:

无论是纸质文件还是内牙科文件,都为基于16S rDNA的根通道微生物分析提供了有效的标本.
末牙仪器采样更容易执行,并被建议作为临床应用的首选方法.

关键词:

亚皮性牙周炎 (apical periodontitis) 是一种牙周炎.污染污染污染的污染是什么微生物组是一个微生物组.这是下一代测序.采样方法采样方法

更多相关视频

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

Published on: December 31, 2017

相关概念视频

Applications of Molecular Taxonomy

Applications of Molecular Taxonomy

Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...