通过 LA-ICP-TOFMS 对生物成像应用的碰撞/反应细胞技术和微滴校准进行先进的元素分析
Sarah Theiner1, Elisabeth Foels2, Gunda Koellensperger1
1Institute of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Waehringer Strasse 38, 1090, Vienna, Austria.
Analytica chimica acta
|November 23, 2024
概括
碰撞/反应电池 (CCT) 模式增强激光除感应合等离子体飞行时间质谱 (LA-ICP-TOFMS) 进行全面的基本生物成像. 这种方法准确地量化了内源和更高质量范围的元素,提高了灵敏度和简化了分析.
科学领域:
- 分析化学 分析化学
- 生物成像是一种生物成像.
- 质谱测量质量谱测量
背景情况:
- 激光除感应合等离子体飞行时间质谱 (LA-ICP-TOFMS) 对于整个质量范围的元素生物成像至关重要.
- 内源元素对细胞金属稳态至关重要,破坏与疾病有关.
- 较高质量范围的元素可以来自标签,纳米粒子或药物,需要精确的检测.
研究的目的:
- 在LA-ICP-TOFMS中评估碰撞/反应细胞 (CCT) 模式在整个质谱的同时元素分析的有效性.
- 用血清参考材料证明LA-ICP-TOFMS方法的准确性.
- 为了评估CCT模式的性能与生物成像应用的标准模式相比.
主要方法:
- 使用激光除感应合等离子体飞行时间质谱 (LA-ICP-TOFMS) 具有或没有碰撞/反应电池 (CCT) 模式.
- 分析的皮克升体积微滴标准和血清参考材料 (Seronorm) 作为微滴沉积.
- 在CCT和标准模式之间对内源性和更高质量范围的元素进行比较量化.
主要成果:
- CCT模式显著优于LA-ICP-TOFMS的标准模式,特别是在易受干扰的内源元件中.
- 对铁和等元素进行了精确的量化,同位素的比率与自然成分相匹配.
- 对于更高质量范围的元素,CCT模式增强了灵敏度 (1.5-2x),而不影响内源元素灵敏度,并消除了后期数据处理.
结论:
- 集成Seronorm血清参考材料微滴到LA-ICPMS生物成像中进行质量控制和验证测量.
- 对于需要在整个质量范围内进行分析的LA-ICP-TOFMS生物成像应用,强烈建议使用CCT模式.
- CCT模式简化了分析程序,并提高了全面的基本生物成像的准确性和灵敏性.
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