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Updated: Jun 6, 2025

Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons
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活细胞中多巴胺的光成像是由独特的合反应触发的

Shaofei Fu1, Jianwen Qiu1, Biaofeng Gu1

  • 1College of Chemistry and Materials Science, Fujian Normal University, Fuzhou, 350117, China.

Analytica chimica acta
|November 23, 2024
PubMed
概括

研究人员开发了一种新的有机光探针,用于检测活细胞中的多巴胺 (DA). 这种方法可以在神经系统疾病的细胞模型中可视化DA水平.

关键词:
细胞成像 细胞成像合反应是一种合反应.多巴胺检测检测多巴胺的检测内源性 HClOO 的内源性光发射探测器进行光.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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