两动物Rotala rotundifolia的生物活性成分在不同的生长阶段和响应表面优化,以提取黄类物质
Shuyi Chu1,2, Zhijun Shi1, Jibo Xiao3,4
1National and Local Joint Engineering Research Center of Ecological Treatment Technology for Urban Water Pollution, Wenzhou University, Wenzhou, 325035, China.
Scientific reports
|November 23, 2024
概括
这项研究表明,浸水的Rotala rotundifolia叶子含有明显高于新兴形式的黄酸盐含量. 确定了最佳的提取条件,以最大限度地提高这种多功能水生植物中的生物活性化合物.
科学领域:
- 植物化学 植物化学
- 植物科学 植物科学
- 水生植物学 水生植物学
背景情况:
- 罗塔拉 (Rotala rotundifolia) 是一种两植物,在中国有传统的药用.
- 它在去除和方面表现出高效率.
- 了解其生物活性成分对于其药用和环境应用至关重要.
研究的目的:
- 为了研究和量化生物活性成分 (黄类,三类,类,类,类) 在潜水和涌现的Rotala rotundifolia的不同组织.
- 使用响应表面方法优化全方位黄的提取条件.
- 为了提供植物化学证据,以更广泛地利用Rotala rotundifolia.
主要方法:
- 在不同生长阶段的R. rotundifolia沉浸和涌现的组织中对生物活性化合物的比较分析.
- 响应表面方法来优化黄酸提取参数 (时间,功率,乙醇度,固体-液体比率).
- 液体染色学-质谱学/质谱学 (LC-MS/MS) 用于黄类物质的鉴定.
主要成果:
- 黄类和三类是占主导地位的化合物,超过了素和类.
- 浸泡的R. rotundifolia的叶子在第30天产生了明显更高的总黄 (270.92毫克/克) 与新出现的形式 (150.45毫克/克) 相比.
- 在沉浸的 (242.20毫克/克在第90天) 和涌现的 (163.09毫克/克在第150天) 形式中,在不同的生长阶段发生了三烯酸含量的峰值.
- 最佳的黄酸提取需要50分钟,333W,79.3%的乙醇和1:60的固体液体比率.
- LC-MS/MS在浸泡的R. rotundifolia提取物中发现了26种黄,在浮现的R. rotundifolia提取物中发现了22种黄.
结论:
- 浸水的罗塔拉轮叶,特别是在植物形态的阶段,是富含全类固醇的丰富来源.
- 三聚胺含量随着生长阶段和植物形式 (沉浸式与涌现式) 显著变化.
- 优化的提取方法为有效分离生物活性黄素提供了基础,支持R. rotundifolia的药用和工业应用.
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