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Updated: Jun 6, 2025

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker
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Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker

Published on: January 3, 2019

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在RAD18催化下形成无化中间体,模仿增殖细胞核抗原PCNA的形成

Liying Zhang1, Zhiheng Deng1, Yunxiang Du1

  • 1New Cornerstone Science Laboratory, Tsinghua-Peking Center for Life Sciences, Ministry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Center for Synthetic and Systems Biology, Department of Chemistry, Tsinghua University, Beijing 100084, China.

Bioorganic & medicinal chemistry
|November 24, 2024
PubMed
概括

这项研究通过在E2-Ub结合物和PCNA等折叠蛋白质基质之间形成二硫化键来扩大泛化研究的化学捕获,从而使新的结构分析成为可能.

关键词:
化学交叉连接质谱学 化学交叉连接质谱学化学蛋白质合成 化学蛋白质合成化学捕获策略 化学捕获策略在PCNA无处不在的化.

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