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在番茄植物中使用酸和离子液功能化单质毛细体柱结合UHPLC-MS/MS和UHPLC-MS/MS的六种细胞因子的快速分析

Huifeng Deng¹, Yanqun Yang¹, Jianbin Ju¹

¹Guangxi Colleges and Universities Key Laboratory of Food Safety and Detection, College of Chemistry and Bioengineering, Guilin University of Technology, Guangxi, 541004, PR China.

Journal of chromatography. A

|November 24, 2024

概括

一个新的单质毛细管柱有效地通过点击聚合来丰富植物中的微量细胞激素. 这种方法可以在番茄器官中进行敏感的检测,有助于植物生长研究.

科学领域:

分析化学分析化学
材料科学材料科学材料科学
植物科学植物科学

背景情况:

细胞因子是调节生长和发育的关键植物激素.
在复杂的植物基质中有效提取和敏感检测细胞激素仍然具有挑战性.

研究的目的:

开发一种新的单立体毛细管柱,以有效丰富细胞因素.
建立一种敏感的分析方法,用于植物器官中细胞激素的确定.

主要方法:

通过硫基-基-基点击聚合制备一个单质毛细体柱 (VPBA-SH-IL) 使用4-维尼尔基酸,离子液体和二乙烯基醇hexakis (((3-mercaptopropionate).
使用柱子进行管内固体相微提取,并与超高性能液态染色学-并体质谱学 (UHPLC-MS/MS) 相结合.

主要成果:

VPBA-SH-IL列显示出出色的透性,化学稳定性和六种细胞因子的高效丰富性 (>95%的效率,0.180.99 mg·m-1容量).
开发的UHPLC-MS/MS方法提供了广泛的线性范围 (0.01010.0 μg·L-1) 和低检测极限 (1.63.3 ng·L-1).
在的番茄样本中,细胞激素的高恢复率 (80.6108%) 和较低的相对标准偏差 (2.48.7%) 得到了实现.

结论:

单立柱VPBA-SH-IL是一种有前途的工具,用于提取和分析复杂植物基质中的微量细胞因子.
这种方法显著推进了植物生长和发育机制的探索,因为它可以准确量化细胞因子.

关键词:

细胞因子是细胞因子的一种.在管内使用的SPME,UHPLC-MS/MS.离子液体是一种离子液体.基酸是什么? 基酸是什么?硫-基的点击聚合.

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