对口腔特征和Erysiphe necator (Schw.) 的比较分析 在多种Vitis sp中抗
M Ramya Sree1, Sanjay K Singh1, Jai Prakash1
1Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India.
概括
葡萄藤粉耐药性涉及诸如沉没的胃口和密集的三角体等物理特征. 这些特征,以及编程细胞死亡 (PCD),限制了病原体Erysiphe necator感染,有助于繁殖工作.
科学领域:
- 植物病理学 植物病理学
- 园艺园艺 园艺园艺
- 遗传学 是一个遗传学.
背景情况:
- 由Erysiphe necator引起的粉状菌对葡萄藤种植构成重大威胁.
- 了解维蒂斯基因型中的耐药性机制对于疾病管理至关重要.
研究的目的:
- 调查口腔和结构特征在维蒂斯对粉状菌的耐药性中的作用.
- 为了确定表现出增强耐药性的特定Vitis基因型.
主要方法:
- 对口腔特征和密度进行显微镜分析.
- 用Erysiphe necator接种病原体和评估感染率.
- 低温扫描电子显微镜 (Cryo-SEM) 用于观察真菌生长.
- 防御反应的分析,包括沉积,表皮状,活性氧物种和编程细胞死亡 (PCD).
主要成果:
- 在Vitis基因型中观察到的口腔特征的显著变化 (沉入与升起,密度,复杂区域).
- 耐药的基因型 (例如V. parviflora,V. jacquemontii,特定杂交物) 显示病原体透率降低,菌根生长有限.
- 耐药基因型采用编程细胞死亡 (PCD) 介导的防御,阻止高达55%的透尝试.
- 物理障碍,如V. parviflora上的密集的三合体和条纹,有助于抵抗性.
- 卡洛斯沉积,表皮状,活性氧物种和快速PCD激活是关键的早期防御机制.
结论:
- 胃和结构特征,包括三合体和,在葡萄酒对粉状菌的抵抗中起着至关重要的作用.
- 编程细胞死亡 (PCD) 和过敏反应对于有效防御Erysiphe necator至关重要.
- 这些发现为培育耐病葡萄品种提供了宝贵的见解.
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