相关实验视频

Updated: Jun 6, 2025

Author Spotlight: Investigating Liver Cancer Pathogenesis Using Patient-Derived Organoids

Author Spotlight: Investigating Liver Cancer Pathogenesis Using Patient-Derived Organoids

Published on: August 18, 2023

糖蛋白酶的结构和功能优化,用于口腔状细胞癌的向生物催化

Wenlu Li¹, Hao Fu¹, Hong Ma¹

¹Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, Henan 45000, China.

International journal of biological macromolecules

|November 24, 2024

概括

优化的葡萄糖蛋白酶对口腔状细胞癌 (OSCC) 治疗有希望. 这种新型治疗有效地减少了瘤生长,诱导了亡,并在临床前模型中抑制了血管生成.

科学领域:

生物化学和分子生物学
在瘤学瘤学.
生物技术是生物技术.

背景情况:

口腔状细胞癌 (OSCC) 仍然是一个重大的全球健康挑战.
新的治疗策略对于改善患者的治疗结果至关重要.
糖蛋白酶代表了一类潜在的治疗剂.

研究的目的:

评估优化糖蛋白酶作为OSCC的新治疗方法的疗效.
描述OSCC细胞系 (SCC-25和HN4) 的稳定性和活力.
在体外和体内评估糖蛋白酶的抗癌作用.

主要方法:

描述SCC-25和HN4细胞系的形态和活力.
再组合糖蛋白酶的表达和净化.
对OSCC细胞的酶活性和细胞毒性的体外评估 (IC50的确定).
使用临床前异种移植模型进行体内评估,分析瘤体积,生长率和分子标记物 (Ki-67,Bcl-2,裂开的酶-3,Bax/Bcl-2比率,CD31).

主要成果:

用糖蛋白酶治疗显著降低了OSCC细胞活力,IC50值为10-15μg/ml.
在体内研究表明,瘤体积和生长速度减少了40-50%.
接受治疗的瘤显示出亡的增加 (降低Ki-67,Bcl-2;增加分离的caspase-3,Bax/Bcl-2比率) 和降低血管生成 (降低微血管密度).

结论:

优化糖蛋白酶疗法有效抑制OSCC瘤的生长.
该疗法诱导了亡并减少了血管生成,这是癌症治疗的关键机制.
这些发现为OSCC治疗中糖蛋白酶的临床应用提供了坚实的基础.

关键词:

细胞灭亡 (apoptosis) 是一种死亡的过程.优化酶的优化酶的优化葡萄糖蛋白酶酶是一种糖蛋白酶.口腔状细胞癌 (OSCC) 是一种瘤的生存能力.

更多相关视频

A Method of Targeted Cell Isolation via Glass Surface Functionalization

A Method of Targeted Cell Isolation via Glass Surface Functionalization

Published on: September 20, 2016

Structural Biology and Analytical Chemistry Approaches for Characterizing C-Glycoside Metabolic Enzymes in Human Gut Microbiota

Structural Biology and Analytical Chemistry Approaches for Characterizing C-Glycoside Metabolic Enzymes in Human Gut Microbiota

Published on: May 23, 2025

相关概念视频

Targeted Cancer Therapies

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...

Catalytically Perfect Enzymes

Catalytically Perfect Enzymes

The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.

Most enzymes...

Cancer Therapies

Cancer Therapies

Cancer therapies are various modes of treatment, such as surgery, radiation therapy, and chemotherapy that are administered to cancer patients.
However, cancer treatments can pose several challenges, as therapies used to kill cancer cells are generally also toxic to normal cells. Moreover, cancer cells mutate rapidly and can develop resistance to chemical agents or radiation therapy. Besides, all types of cancer cells may not respond to the same therapy. Some cancer cells respond to one...

Protein Glycosylation

Protein Glycosylation

Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...