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相关概念视频

What is Gene Expression?01:36

What is Gene Expression?

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A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
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相关实验视频

Updated: Jun 6, 2025

mRNA Interactome Capture from Plant Protoplasts
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转录基因分析了叶子的编码体使用模式和基因表达特征.

Zhanjun Wang1,2,3, Jie Li1, Xuyuan Liu4

  • 1School of Biology and Food Engineering, Hefei Normal University, Hefei, Anhui, China.

BMC plant biology
|November 24, 2024
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概括

叶状突起表现出受自然选择影响的代码使用偏差 (CUB),影响基因表达. 了解这种偏差是改善基因表达和开发转基因叶子应用程序的关键.

关键词:
在Codon的使用中,存在偏见.表达式分析表达式分析叶子的树苗是叶子的序列重新组装 序列重新组装

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科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 植物科学 植物科学

背景情况:

  • 叶 (Euphorbia esula) 是一种具有药用和能量潜力的宝贵草本.
  • 子使用偏差 (CUB) 是由进化塑造的基因组特征,对分子繁殖至关重要.
  • 了解CUB的叶子可以优化生物技术应用的基因表达.

研究的目的:

  • 为了分析叶子的基因组中的子使用偏差.
  • 确定影响CUB的因素及其与基因表达的关系.
  • 为在叶子中选择有效的外源表达系统奠定基础.

主要方法:

  • 使用TransDecoder来识别编码区域的转录组数据分析.
  • 全基因组分析以检测基因组合偏差和高频密码子.
  • 对子使用频率和基因表达水平的比较分析.

主要成果:

  • 叶子突起基因组显示A/T偏差,特别是在末端位置,确定了七个高频密码.
  • 自然选择和其他因素在塑造CUB方面占主导地位,以及突变压力和基质组成.
  • 在CUB和基因表达水平之间观察到显著的正相关性;高度表达的基因显示出更大的CUB和趋向于终端G/C基.

结论:

  • 叶状的使用偏差受到进化压力的显著影响,主要是自然选择.
  • 叶状的高度表达的基因表现出明显的CUB模式,有利于终端G / C基.
  • 这项研究为优化基因表达和开发转基因叶提供了基础.