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6-甘醇和阿斯塔桑丁可以减轻酸在猪卵细胞成熟过程中的作用

Hussain Ahmad Saeed¹, Rabia Sabir², Xinyue Lu¹

¹College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.

Reproduction in domestic animals = Zuchthygiene

|November 25, 2024

概括

酸 (SA) 的高含量会损害卵细胞的成熟和生殖成功. 抗氧化剂6-甘醇 (6-G) 和阿斯丁 (AX) 保护了猪卵细胞免受SA诱导的脂毒性,提高了活力和发育能力.

科学领域:

生殖生物学生殖生物学
细胞的新陈代谢
毒理学毒理学毒理学

背景情况:

增加的非化脂肪酸 (NEFAs),特别是酸 (SA),会对卵细胞的成熟和质量产生负面影响.
SA诱导的脂毒性破坏细胞代谢,导致卵细胞功能受损和潜在的生殖失败.

研究的目的:

为了研究SA在体成熟 (IVM) 过程中对猪卵细胞的脂毒作用.
评估6-甘醇 (6-G) 和阿斯丁 (AX) 对抗SA诱导的损伤的保护潜力.

主要方法:

猪积卵细胞复合物 (COCs) 暴露于不同SA度 (25-250μM).
评估了6-G (5-15 μM) 和AX (2.5 μM) 在减轻SA (150 μM) 毒性的有效性.
评估了卵细胞活力,极体挤出 (PBE) 和累积细胞扩张指数 (CCEI).

主要成果:

150μM的SA显著降低了卵细胞活力,PBE和CCEI,证实了它对发育能力的有害影响.
与SA单独相比,与6-G (10μM) 或AX联合治疗显著增加了卵细胞活力和PBE.
抗氧化剂治疗改善了脂质平衡和细胞抗氧化防御.

结论:

由SA引起的脂毒性会损害卵细胞质量和生殖能力.
6-甘醇和阿斯塔桑丁可以显著保护SA诱导的卵细胞损伤.
用抗氧化剂优化卵细胞微环境可以提高卵细胞质量,从而改善生殖技术.

关键词:

6 - 甘醇的使用阿斯塔克桑丁是一种阿斯塔克桑丁.在体外成熟的成熟.卵子细胞卵子细胞猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪乙醇酸是什么? 乙醇酸是什么?

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