胰岛素-转-补充剂改善了猪胚胎的体外生产
Juan Lin1, Zhuqing Ji1, Shenming Zeng1
1National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding, and Reproduction of the Ministry of Agriculture and Rural Area, College of Animal Science and Technology, China Agricultural University, Beijing100193, P.R. China.
Zygote (Cambridge, England)
|November 25, 2024
概括
在成熟和培养基中补充胰岛素-转移素- (ITS) 显著提高了猪胚胎的生产. 这提高了卵细胞质量,增加了胚胎细胞的数量和细胞数量,以实现高效的体外发育.
科学领域:
- 生殖生物学 生殖生物学
- 动物科学动物科学
- 生物技术是生物技术.
背景情况:
- 猪胚胎的体外生产涉及复杂的步骤:体外成熟 (IVM),体外受精 (IVF) 和体外培养 (IVC).
- 挑战包括细胞质成熟不充分, zona pellucida反应缓慢,培养条件低于最佳,所有这些都会影响猪胚胎生产效率.
- 之前的研究表明,在IVM或IVC介质中补充胰岛素-转- (ITS) 可以增强卵细胞成熟,减少多精子并促进胚胎发育.
研究的目的:
- 调查胰岛素-转- (ITS) 补充剂在体外成熟 (IVM) 和体外培养 (IVC) 介质中对猪胚胎生产的综合作用.
- 阐明ITS对猪卵细胞质量和随后胚胎发育的影响.
主要方法:
- 在IVM和IVC介质中补充1.0%的胰岛素-转移素- (ITS),用于猪胚胎生产.
- 评估卵细胞细胞质成熟标志物,包括累积细胞扩张,线粒体膜潜力,ATP含量和活性氧物种 (ROS) 水平.
- 对随后的胚胎发育进行评估,重点关注胚胎细胞形成率和胚胎细胞数量.
主要成果:
- 在IVM/IVC介质中补充1.0%的ITS显著促进了累积细胞扩张.
- ITS治疗导致线粒体膜潜力增加,ATP含量增加,并在成熟的猪卵细胞中降低ROS水平.
- 在ITS补充的组中,胚胎细胞率和胚胎细胞内的总细胞数显著改善.
结论:
- 用1.0%的胰岛素转移素 (ITS) 补充体外成熟和体外培养基可以有效地提高猪卵细胞质量.
- 在IVM和IVC介质中使用ITS可以改善关键的发育参数,包括囊胚形成和细胞数量.
- 这项研究表明,在IVM/IVC介质中补充1.0%的ITS是一种可行的策略,可以提高猪胚胎生产的整体效率.
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