相关实验视频

Updated: Jun 6, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

特定的肠道微生物组特征预测了急性缺血性中风的风险

Shicheng Yu¹, Jiayu Shi¹, Gaojie Yu¹

¹Department of Geriatrics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.

Frontiers in aging neuroscience

|November 25, 2024

概括

肠道微生物群在短暂缺血性攻击 (TIA) 和急性缺血性中风 (AIS) 患者之间存在显著差异. 特定的细菌,如乳杆菌和链球菌可以作为生物标志物来区分TIA和AIS.

科学领域:

微生物组研究的研究.
神经学神经学
胃肠病学胃肠病学

背景情况:

在急性缺血性中风 (AIS) 患者中观察到肠道微生物群的改变,随着疾病的进展变化加剧.
过渡性缺血性攻击 (TIA) 进展和AIS之间的关系仍然不完全理解.
这项研究调查了TIA,AIS和健康对照 (HC) 之间的微生物差异.

研究的目的:

为了阐明TIA,AIS和HC组之间的微生物差异.
探索疾病进展和肠道微生物群组成之间的关联.
确定潜在的肠道细菌生物标志物,以区分TIA和AIS.

主要方法:

从TIA (n=28),AIS (n=235) 和HC (n=75) 组收集了便样本.
16S rRNA基因测序用于微生物分析.
线性差异分析效果大小和接收器运行特征 (ROC) 曲线用于识别特征性微生物群和评估诊断价值.

主要成果:

在TIA,AIS和HC组中发现了显著的肠道微生物差异.
在AIS患者中,乳杆菌和链球菌的丰度较高,而Butyricicoccaceae和Lachnospiraceae的丰度较低_UCG-004.
乳杆菌和链球菌的丰度与HDL有负相关性,而乳杆菌与同型半氨酸 (Hcy) 有正相关性. Lachnospiraceae_UCG-004的丰度与Hcy和D-二次体有负相关性.
使用Streptococcus和Lactobacillus的ROC模型有效地区分了TIA和AIS (AUC分别为0.699和0.626).

结论:

明显的肠道细菌变化与TIA到AIS的进展有关.
特定的肠道细菌,包括Lactobacillus和Streptococcus,显示出作为疾病进展的预测生物标志物的潜力.
这些发现可能有助于开发基于微生物组的诊断工具,用于早期检测AIS.

关键词:

急性缺血性中风是急性缺血性中风.生物标志物生物标志物我们的肠道微生物群.预测预测预测预测过渡性缺血性攻击是短暂的

更多相关视频

Investigating Alterations in Caecum Microbiota After Traumatic Brain Injury in Mice

Investigating Alterations in Caecum Microbiota After Traumatic Brain Injury in Mice

Published on: September 19, 2019

Murine Fecal Isolation and Microbiota Transplantation

Murine Fecal Isolation and Microbiota Transplantation

Published on: May 26, 2023

相关概念视频

Anatomy of the Intestines

Anatomy of the Intestines

Although digestion of proteins, carbohydrates, and lipids may begin in the stomach, it is completed in the intestine. The absorption of nutrients, water, and electrolytes from food and drink also occurs in the intestine. The intestines can be divided into two structurally distinct organs—the small and large intestines.
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...