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Updated: Jun 6, 2025

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension
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[Expi293F细胞中重链抗体可变域的高表达,具有优化的信号和编码子]

Shuzhen Tan1, Hu Dong1, Songjia Pan1

  • 1State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730000, Gansu, China.

Sheng wu gong cheng xue bao = Chinese journal of biotechnology
|November 25, 2024
PubMed
概括

优化信号和编码子显著增强Expi293F细胞中重链抗体 (VHH) 生产的可变域. 这种方法提高了VHH的产量,并保持了治疗应用的结合活性.

关键词:
代码子优化代码子优化表达方式表达方式表达方式表达信号是一种信号.重链抗体 (VHH) 的可变域

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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