[通过CRISPR/Cas9在HEK293细胞中的RIG-I被淘汰]
Ziyi Chen1, Yirong Wu1, Yuting Zhang1
1College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, Zhejiang, China.
Sheng wu gong cheng xue bao = Chinese journal of biotechnology
|November 25, 2024
概括
通过CRISPR/Cas9对HEK293细胞的网红酸诱导基因I (RIG-I) 淘汰会影响I型干扰素信号传递. RIG-I淘汰细胞显示了MDA5和IFNβ1等关键因子的改变表达,影响了细胞活力.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
相关概念视频
CRISPR
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR/Cas9 Genome Editing
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...


