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相关概念视频

¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied first.
Mass Spectrum01:23

Mass Spectrum

A mass spectrum is the graphical representation of the relative abundance of the charged fragments in an analyte plotted against their mass-to-charge ratio (m/z). The plot's x-axis represents the ratio of the mass of the charged fragment to the number of charges it carries. The y axis of the plot represents the relative abundance of each charged species. The relative abundance is calculated from the signal intensity of each charged species recorded at the detector. The most intense signal (the...

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相关实验视频

Updated: Jun 17, 2026

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

104.8K

单基m6A甲基化可视化和量化

Qiushuang Zhang1,2,3, Yicong Dai1,2, Xucong Teng2,4

  • 1Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing, 100084, China.

Angewandte Chemie (International ed. in English)
|November 25, 2024
PubMed
概括

研究人员开发了一种名为TARS的新方法,以单基分辨率在单细胞内可视化和量化N6-甲基氨酸 (m6A) RNA修饰,克服了以前的成像限制.

关键词:
这是一种N6-甲基氨酸.基因组RNA的修饰是RNA的修饰.m6A量化的量化单一基准解决方案的解决方案一个单细胞成像技术.

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相关实验视频

Last Updated: Jun 17, 2026

DNA Methylation: Bisulphite Modification and Analysis
12:34

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Published on: October 21, 2011

104.8K
Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

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A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
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科学领域:

  • 分子生物学分子生物学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 在RNA生物学,RNA生物学.

背景情况:

  • N6-甲基氨酸 (m6A) 是最丰富的RNA修饰,在各种细胞过程中发挥关键作用.
  • 精确检测和量化m6A对于了解疾病机制和开发治疗方法至关重要.
  • 目前的方法缺乏在单基分辨率下进行细胞内m6A现场可视化的能力.

研究的目的:

  • 开发一种用于精确可视化和量化单细胞内m6ARNA修饰的新方法.
  • 为了在特定的RNA位点实现单基分辨率成像,对腺素 (A) 和N6-甲基腺素 (m6A) 进行成像.

主要方法:

  • 介绍TadA8.20辅助的N6-甲基氨酸RNA成像在单基分辨率 (TARS) 方法.
  • 应用TARS用于现场成像和量化MALAT1 lncRNA和CCND1 mRNA中的m6A修饰.
  • 在HeLa细胞和乳腺癌细胞系中进行验证.

主要成果:

  • 在单个单元中,TARS可在单基分辨率下精确可视化和量化m6A修改.
  • 在MALAT1 lncRNA和CCND1 mRNA上绘制和量化m6A方面表现出高的特异性和效率.
  • 在特定的RNA位点成功可视化了A和m6A形式.

结论:

  • TARS是一个新而强大的工具,用于推进m6ARNA修饰研究.
  • 提供对单基层的m6A修改提供准确和详细的见解.
  • 有助于更深入地了解m6A在生理和病理条件中的作用.