Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

8.2K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
8.2K
Histone Modification02:32

Histone Modification

13.1K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
13.1K
Eukaryotic Transcription Inhibitors01:52

Eukaryotic Transcription Inhibitors

9.8K
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
9.8K
Epigenetic Regulation01:37

Epigenetic Regulation

3.0K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.0K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

7.3K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.3K
Heterochromatin02:38

Heterochromatin

11.2K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
11.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

ROS-SUMO Crosstalk in Oxidative Stress: Disease Mechanisms and Reproductive Health.

Antioxidants (Basel, Switzerland)·2026
Same author

Auto-sumoylation of the yeast Ubc9 E2 SUMO-conjugating enzyme extends cellular lifespan.

Nature communications·2025
Same author

HSD17B4 deficiency causes dysregulation of primary cilia and is alleviated by acetyl-CoA.

Nature communications·2025
Same author

Yeast Small Ubiquitin-Like Modifier (SUMO) Protease Ulp2 is Involved in RNA Splicing.

Development & reproduction·2024
Same author

Carnitine Protects against MPP<sup>+</sup>-Induced Neurotoxicity and Inflammation by Promoting Primary Ciliogenesis in SH-SY5Y Cells.

Cells·2022
Same author

SUMOylation and Major Depressive Disorder.

International journal of molecular sciences·2022
Same journal

Vigorous Physical Activity Mitigates Susceptibility to Obesity Associated with Risk Genotypes of <i>FTO</i> and <i>MC4R</i>, and <i>SREBF1</i> Is Hypermethylated: A Cross-Sectional Pilot Study.

Epigenomes·2026
Same journal

Remodelling of miRNA Regulatory Landscape During West Nile Virus (WNV) Infection.

Epigenomes·2026
Same journal

The Role of Epigenetics in Corneal Fibrosis.

Epigenomes·2026
Same journal

Dual Functionality of miRNAs During HIV Infection: From Viral Genome Suppression to Immune Response Modulation.

Epigenomes·2026
Same journal

Nuclear Transfer Perturbs Genomic Balance.

Epigenomes·2026
Same journal

5mC and 6mA DNA Methylation in the Fungal Kingdom: From Genome Defense to Epigenetic Regulation.

Epigenomes·2026
查看所有相关文章

相关实验视频

Updated: Jun 6, 2025

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

6.4K

基质子修改途径 抑制密码转录

Hong-Yeoul Ryu1,2

  • 1KNU G-LAMP Project Group, KNU Institute of Basic Sciences, Kyungpook National University, Daegu 41566, Republic of Korea.

Epigenomes
|November 25, 2024
PubMed
概括
此摘要是机器生成的。

像H3K36和H3K4甲基化这样的质子修饰阻止了神秘的转录,这是可能损害细胞的意外基因表达. 了解这些表观遗传标记是保持转录忠实性和开发新疾病治疗的关键.

关键词:
在H3K36甲基化过程中.在H3K4甲基化过程中.在Rpd3S HDAC复合体中.设置3 HDAC 复杂的复合体隐秘的转录 转录的隐秘方式

更多相关视频

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

1.4K
Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
10:09

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Published on: January 26, 2018

7.4K

相关实验视频

Last Updated: Jun 6, 2025

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

6.4K
In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

1.4K
Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
10:09

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Published on: January 26, 2018

7.4K

科学领域:

  • 表观遗传学和分子生物学
  • 基因规则 基因规则
  • 染色体生物学 染色体生物学

背景情况:

  • 密码转录涉及来自非正规基因组部位的意外基因表达,可能导致异常蛋白质和破坏细胞功能.
  • 基因组蛋白修饰是影响染色质结构和基因表达的关键表观遗传调节剂.
  • 特定的基因素甲基化标记,H3K36和H3K4,与控制转录有关.

研究的目的:

  • 探索基因组修饰,特别是H3K36和H3K4甲基化在调节密码转录中的作用.
  • 阐明这些基因素标记维持转录忠实性和细胞完整性的机制.
  • 突出与基因表达失调相关的疾病的潜在治疗途径.

主要方法:

  • 这篇意见稿回顾了关于激素修饰和密码转录的现有文献.
  • 专注于H3K36三甲基化和H3K4二甲基化的功能作用.
  • 讨论了像Rpd3S和Set3这样的基因组脱乙酶 (HDAC) 复合物的参与,以调节染色体状态.

主要成果:

  • H3K36三甲基化招募了Rpd3S HDAC复合体,促进了闭合色素,并防止了基因体内的神秘启动.
  • 在H3K4二甲基化,无处不在化和化之间的交叉声调用Set3 HDAC复合体,抑制基因体乙化和神秘转录.
  • 这些组素修改集体保持转录忠实性.

结论:

  • 基质子修饰,特别是H3K36和H3K4甲基化,是抑制密码转录的关键调节者.
  • 基因素标记和HDAC复合体之间的相互作用对于保持细胞完整性和适当的基因表达至关重要.
  • 对这些机制的进一步研究可能为与年龄有关的疾病和其他疾病提供新的治疗策略.