在丝虫,Bombyx mori中的Gynandromorphic脂肪体的细胞自主性差异化转录体证据
Fumiko Yamamoto1, Takeshi Yokoyama2, Yan Su3
1Anicom Pafe, Inc., Sumitomo Fudosan, 8-17-1, Shinjyuku, Shinjyuku-ku 160-0023, Tokyo, Japan.
Journal of developmental biology
|November 25, 2024
概括
在丝虫中,脂肪体中的大多数性别特异性基因表达是细胞自主的,这意味着它是细胞自主的.
科学领域:
- 发展生物学 发展生物学
- 遗传学 是一个遗传学.
- 昆虫分子生物学 昆虫分子生物学
背景情况:
- 昆虫的性别决定传统上假定细胞自主控制.
- 新出现的证据表明,通过外部因素对二次性特征进行非细胞自主调节.
- 了解细胞自主和非细胞自主调节之间的平衡对于昆虫的发育至关重要.
研究的目的:
- 调查二次性性特征发展的细胞自主与非细胞自主调节的程度.
- 为了分析 gynandromorphic 丝虫 (Bombyx mori) 的性二形转录组.
- 确定脂肪体中性别差异表达基因 (S-DEGs) 的调节机制.
主要方法:
- 对来自Bombyx mori.mo突变菌株的妇形个体的分析.
- RNA测序 (RNA-seq) 用于比较雄性和雌性幼虫脂肪体之间的转录组.
- 使用Feminizer (Fem) 基因表达的ZZ和ZW细胞比例的量化.
- 估计和观察到的S-DEG表达水平之间的相关性分析.
主要成果:
- 在脂肪体中确定了232个性别差异表达基因 (S-DEGs).
- 基于 gynandromorphs 中 ZZ/ZW 细胞比例的估计 S-DEG 表达.
- 在估计和观察到的表达水平之间发现了强烈的相关性 (207/232 S-DEGs).
- 证明脂肪体中的大多数S-DEGs以细胞自主的方式受到调节.
结论:
- 丝虫脂肪体中的性二态转录组主要是细胞自主调节的.
- 这一发现支持了在昆虫中细胞自主性决定二次性特征的经典模型.
- 突出了细胞内在机制在昆虫中建立性二态化的重要性.
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