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Actin and myosin or actomyosin filaments also play a significant role in cells other than those involved in muscle contraction (which occurs within the sarcomere of muscle cells). The mechanism of non-muscle cell contractile bundles was first observed in Dictyostelium and Acanthamoeba. In non-muscle cells, two bundles are commonly found: stress fibers and actomyosin adherence belts. These contractile bundles are smaller and less organized than the ones found in muscle cells. They  are held...
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Fragmenting Bulk Hydrogels and Processing into Granular Hydrogels for Biomedical Applications
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工程颗粒状基体没有粒子间交联,以支持多细胞组织.

Natasha L Claxton1, Melissa A Luse2, Brant E Isakson2,3

  • 1Department of Biomedical Engineering, University of Virginia, Charlottesville, Virginia 22903, United States.

ACS biomaterials science & engineering
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PubMed
概括

这项研究引入了用于高级3D细胞培养的新型,非交叉链接的颗粒状水凝. 这些材料支持细胞自我组织和组织形成,对于开发更好的体外模型至关重要.

关键词:
蜂连接 蜂连接 蜂连接内皮细胞是内皮细胞.纤维细胞的细胞.有颗粒状的水凝.微凝是一种微凝.形态发生 (morphogenesis) 是一种形态的产生.宽容,宽容,宽容,宽容,宽容,宽容.

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科学领域:

  • 生物材料科学 生物材料科学
  • 组织工程是组织工程.
  • 细胞生物学 细胞生物学

背景情况:

  • 开发先进的三维 (3D) 组织结构对于创建精确模仿生物系统的体外模型和工程组织至关重要.
  • 允许细胞行为如增殖,形态发生和运动的材料是形成功能性组织结构的关键.
  • 虽然已经建立了交叉连接的颗粒状水凝,但支持细胞自我组织的无交叉连接的颗粒状系统尚未得到探索.

研究的目的:

  • 开发和描述一种基于聚乙烯糖醇的颗粒状水凝系统,没有3D细胞培养的粒子间交叉链接.
  • 为了研究材料特性,如多孔性和细胞粘合性连接体,对水凝内的细胞网络形成的影响.
  • 评估非交联颗粒状水凝在支持血管生成和组织成熟等形态遗传过程方面的潜力.

主要方法:

  • 制造以聚乙烯糖醇为基础的颗粒状水凝,颗粒直径小于40微米.
  • 描述水凝特性,包括散装压力放松行为和与细胞培养微器件的兼容性.
  • 在水凝系统内共培养内皮细胞和纤维细胞,操纵交叉连接,多孔性和RGD连接体的结合.

主要成果:

  • 开发的颗粒式水凝系统显示了大量的减压特性和持续细胞培养的稳定性.
  • 增加的水凝多孔性显著增强了细胞网络的形成 (例如,血管生成).
  • 在这种特定系统中,将细胞粘附性配体 (RGD) 纳入细胞网络形成产生了负面影响.

结论:

  • 没有交叉连接的颗粒状水凝是支持细胞自我组织和组织发育的有希望的材料平台.
  • 材料设计参数,特别是孔隙度,可以调整以促进3D细胞培养中的特定形态遗传结果.
  • 这项研究为设计用于组织工程和体外建模应用的先进生物材料提供了宝贵的见解.