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来自无皮大麦的HvbHLH132的子宫外表达减少了转基因Arabidopsis thaliana的寒冷耐受性

Yilei Hao^1,2, Jing Su³, Yongmei Cui^4,5

¹State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, 810016, Qinghai, China.

Plant cell reports

|November 25, 2024

概括

在大麦中过度表达HvbHLH132基因会降低Arabidopsis幼苗的冷却和结耐受性. 这项研究确定了141个HvbHLH基因,并突出了HvbHLH132基因.

科学领域:

植物分子生物学植物分子生物学
基因组学就是基因组学.
压力生理学压力生理学

背景情况:

基本螺旋环-螺旋环 (bHLH) 转录因子调节植物的重要过程.
在无大麦 (Hordeum vulgare) 中的bHLH基因家族的特征并不清楚.
了解bHLH功能对于提高作物应激弹性至关重要.

研究的目的:

为了在无大麦中对HvbHLH基因家族进行全基因组识别.
为了研究HvbHLH132在应对寒冷压力的功能.
评估HvbHLH132对植物耐寒性的影响.

主要方法:

全基因组识别和 HvbHLH 基因的亚家族分类.
在HVbHLH促进区域中分析cis作用元素.
在寒冷压力下表达特征和亚细胞局部化研究.
在Arabidopsis thaliana中HvbHLH132的过度表达,以评估耐寒表型和基因表达.

主要成果:

在无大麦中确定了141个HvbHLH基因,分为12个子家族.
在冷应力下,HvbHLH132显示出显著的诱导和差异性表达.
在Arabidopsis中HvbHLH132的过度表达,在幼苗阶段降低了冷却和结耐受性.
HvbHLH132的过度表达导致了不利的生化变化和降低了对冷反应 (COR) 基因的诱导.

结论:

HvbHLH132负面调节了Arabidopsis幼苗的冷却和冷耐受性.
HvbHLH基因家族在植物对寒冷压力的反应中发挥作用.
HvbHLH132是影响植物耐寒机制的关键调节剂.

关键词:

HvbHLH基因家族的基因家族这就是HVBHLH1322.阿拉比多普西斯 (Arabidopsis) 是一种植物.耐寒性耐寒性耐寒性耐寒性

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