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全基因组关联研究确定了参与外源ATP利用和调节Brassica napus中含量的BnaPAP17基因

Hao Li^1,2, Haijiang Liu³, Chuang Wang²

¹National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.

Plant cell reports

|November 25, 2024

概括

研究人员确定了与Brassica napus.17中根部分泌的酸酶活性相关的BnaPAP17基因. 这些基因增强了从有机来源吸收的能力,这对于在缺乏的情况下的作物产量至关重要.

科学领域:

植物分子生物学植物分子生物学
农业科学农业科学
遗传学是一个遗传学.

背景情况:

土壤缺乏限制了油菜 (Brassica napus) 的作物产量和质量.
有机 (Po) 是主要的土壤池,需要通过酸酸酶 (APases) 溶解为无机 (Pi),以便植物吸收.
根分泌的APases (SAP) 活性对于Po利用至关重要,但其在B. napus中的遗传基础尚不清楚.

研究的目的:

通过全基因组关联研究 (GWAS) 在B. napus的Pi缺陷下识别与SAP活动相关的遗传位置.
研究候选基因,特别是BnaPAP17s在Po利用和植物含量的作用.

主要方法:

在350个B. napus加入中执行了GWAS,使用超过450万个SNP来识别与Pi缺陷下的SAP活动相关的位点.
选择了BnaA01.PAP17候选基因及其对应基因,用于表达分析,亚细胞定位和通过过度表达研究进行功能验证.

主要成果:

确定了35个与SAP活动相关的显著SNP,其中BnaA01.PAP17被确定为候选基因.
BnaPAP17s表现出Pi缺陷诱导的表达模式,与Pi度相反相关,表明它们作为Pi标记基因的作用.
过度表达BnaPAP17s增强了SAP活动,增加了植物含量,而ATP是唯一的来源.

结论:

BnaPAP17基因与根部分泌的APases活性有关,并由Pi-缺乏引起.
BnaPAP17s有助于细胞外Po利用,增强植物吸收,并可能改善低土壤中的作物性能.

关键词:

在BnaPAP17上布拉斯卡纳普斯 (Brassica napus) 是一种植物.全基因组关联研究 (GWAS)有机 (Po) 有机 (Po)根部分泌的酸酸酶.

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