描述,优化和扩大潜水Inonotus hispidus菌发酵的规模,以提高生物质和多糖的生产
Ke Shen1, Yuanshuai Liu2, Liyan Liu1
1School of Food Science & Engineering, South China University of Technology, Guangzhou, 510640, China.
Applied biochemistry and biotechnology
|November 25, 2024
概括
优化注射体形态显著增强了Inonotus hispidus的浸泡发酵. 分散的菌根碎片产生更高的生物质和多糖,这对这种食用和药用真菌至关重要.
科学领域:
- 生物技术是生物技术.
- 菌类学 菌类学是指菌类学.
- 食品科学 食品科学 食品科学
背景情况:
- Inonotus hispidus是一种食用和药用真菌,含有有价值的生物活性化合物.
- 浸泡菌发酵是生产真菌生物质和代谢物的关键过程.
- 控制注射器特性对于高效的发酵至关重要.
研究的目的:
- 通过控制注射器形态来开发一种有效的潜水发酵策略,用于Inonotus hispidus.
- 为了比较分散的菌根碎片与集成的块作为注射剂的影响.
- 优化注射剂的制备和发酵条件,以最大限度地提高生物质和多糖的产量.
主要方法:
- 分散的菌根碎片和聚合的菌根块作为注射剂的比较.
- 使用同质化 (26,000rpm,3分钟) 优化注射剂制备.
- 在5L和100L混合发酵器中进行了扩大规模的研究.
主要成果:
- 分散的菌根碎片导致了更短的滞后期,更高的生物质产量和更均的颗粒.
- 在100升发酵器中优化了注射液和料批发发酵,实现了22.23g/L生物质,3.31g/L外聚糖 (EPS) 和5.21g/L细胞内多糖 (IPS).
- 发酵的I.hispidus菌含有丰富的蛋白质,食纤维和有益于健康的多糖.
结论:
- 无菌体的特征 (年龄,形态,聚合) 显著影响潜水发酵的生产力.
- 优化的注射体形态和食批量策略对大规模生产I. hispidus生物质和多糖有效.
- 这项研究提供了一种有效的策略,通过发酵来利用Inonotus hispidus的营养和药用潜力.
更多相关视频
14:53Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
11.2K
06:24Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
Published on: December 15, 2017
10.0K
相关概念视频
Microbial Fermentation
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
Bioreactor Controls-III
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
Scale-Up Processes
The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...
Upstream Processing
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
Production of Alcohol
Continuous fermentation is a key strategy in industrial ethanol production, particularly when efficiency, scalability, and high yields are essential. This approach allows for uninterrupted operation and optimized resource utilization. The primary feedstock, corn starch, undergoes enzymatic hydrolysis facilitated by α-amylase and glucoamylase. These enzymes break down the starch into fermentable sugars such as glucose, which are readily assimilated by fermentative microorganisms.Fermentation...
Production of Organic Acids
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
