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基于滴滴的单细胞3'mRNA测序马尔堡病毒感染样本

Michelle Meyer1,2, Palaniappan Ramanathan3,4,5

  • 1Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA. mnmeyer@utmb.edu.

Methods in molecular biology (Clifton, N.J.)
|November 25, 2024
PubMed
概括

这项研究详细介绍了非人类灵长类动物中马尔堡病毒感染的单细胞RNA测序协议. 该方法允许对病毒与宿主相互作用进行高分辨率分析,即使是高制性病原体.

关键词:
10× Chromium 下一个宝石滴滴序列测序 滴滴序列测序是指滴滴序列的测序.菲洛病毒是一种病毒.马尔维 (MARV) 是一个名字.单细胞RNA测序的一个细胞.文字转录学 (Transcriptomics) 是一个学科.

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RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...