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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Updated: Jun 6, 2025

Monitoring Spatial Segregation in Surface Colonizing Microbial Populations

Monitoring Spatial Segregation in Surface Colonizing Microbial Populations

Published on: October 29, 2016

使用远红色化探针揭示了生物膜分散模式.

Jojo A Prentice¹, Sandhya Kasivisweswaran¹, Robert van de Weerd^1,2,3

¹Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania, United States of America.

|November 25, 2024

概括

研究人员开发了一种新的成像方法来研究细菌如何从生物膜中分散. 这项技术揭示了细菌分散模式的新细节,并确定了不分散的细胞.

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Concurrent Quantification of Cellular and Extracellular Components of Biofilms

Concurrent Quantification of Cellular and Extracellular Components of Biofilms

Published on: December 10, 2013

Methods for Characterizing the Co-development of Biofilm and Habitat Heterogeneity

Methods for Characterizing the Co-development of Biofilm and Habitat Heterogeneity

Published on: March 11, 2015

相关实验视频

Last Updated: Jun 6, 2025

Monitoring Spatial Segregation in Surface Colonizing Microbial Populations

Monitoring Spatial Segregation in Surface Colonizing Microbial Populations

Published on: October 29, 2016

Concurrent Quantification of Cellular and Extracellular Components of Biofilms

Concurrent Quantification of Cellular and Extracellular Components of Biofilms

Published on: December 10, 2013

Methods for Characterizing the Co-development of Biofilm and Habitat Heterogeneity

Methods for Characterizing the Co-development of Biofilm and Habitat Heterogeneity

Published on: March 11, 2015

科学领域:

微生物学微生物学
细胞生物学细胞生物学
生物物理学的生物物理.

背景情况:

细菌形成生物膜,复杂的社区对于殖民和感染至关重要.
来自生物膜的分散对于细菌的传播至关重要,但在单细胞水平上了解得很少.
传统成像方法的局限性阻碍了在密集,缺氧的生物膜中分散的研究.

研究的目的:

开发和应用一种新的细胞标记策略,用于高分辨率,长期对细菌生物膜分散的成像.
为了研究单细胞机制和生物膜分散在*Vibrio cholerae*的空间动态.
在分散过程中识别新的微尺度模式和细胞行为.

主要方法:

开发了一种细胞标记策略,使用素激活蛋白 (FAP) 和远红色染料用于生物膜中的功能成像.
利用长期成像来描述*Vibrio cholerae*生物膜中前所未有的分辨率的分散动态.
分析了分散模式,包括细胞离开,生物膜压缩和区域细胞运动异质性.

主要成果:

分散开始于生物膜外围,大约25%的细胞没有分散.
在分散过程中观察到的新型微尺度模式包括生物膜压缩和异质细胞运动.
变异的分散速度变化的突变体显示了减弱或同质化的分散模式,影响了当地的机械特性.

结论:

素激活蛋白 (FAPs) 为复杂环境中的高分辨率微生物动态研究提供了强大的工具.
描述了细菌生物膜分散和病原体传播的机制的基本见解.
揭示了以前未被观察到的单细胞行为和控制生物膜分散的空间模式.