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基于关节炎活动和局部皮质类固醇使用的JIA儿童的候选催性尿道炎生物标志物

Ilaria Maccora^1,2, Mekibib Altaye³, Kenneth D Greis⁴

¹Department of Neurosciences, Psychology, Drug Research and Child Health (NEUROFARBA), University of Florence, Florence, Italy.

Ocular immunology and inflammation

|November 25, 2024

概括

眼生物标志物可以帮助在患有青少年异常性关节炎 (JIA) 的儿童中检测尿膜炎. 眼中的特定蛋白质区分了与JIA相关的脑膜炎 (JIA-U) 和没有脑膜炎的JIA (JIA-no-U).

科学领域:

眼科医生眼科眼科
免疫学免疫学免疫学
蛋白质组学是指蛋白质组学.

背景情况:

尿道炎是一种与视网膜色素表皮质 (RPE) 和视网膜血屏障 (BRB) 相关的炎症性眼病.
根据当前的临床因素,预测青少年异常性关节炎 (JIA) 膜炎风险具有挑战性.
眼液提供了一个非侵入性的来源,用于识别疾病的生物标志物.

研究的目的:

在被诊断为JIA的儿童中,识别与子宫炎相关的基于眼的蛋白质标记物.
探索潜在的非侵入性生物标志物用于早期检测JIA相关性脑膜炎 (JIA-U).

主要方法:

从患有JIA-U和JIA-no-U的儿童身上采集了眼,使用了希尔默带.
使用定量蛋白质组学 (iTRAQ和TMT) 和纳米LC-MS/MS来分析蛋白.
差异性蛋白质表达被统计分析,考虑关节炎活性和皮质类固醇使用.

主要成果:

在JIA-U和JIA-no-U患者之间,13种蛋白质的表达有差异.
阿尔法-2-巨型球蛋白,阿波利波蛋白A1,S100A9,哈普托格洛宾和跨甲状腺素始终在区分这些群体.
STRING分析将这些蛋白与RPE,BRB和炎症通路联系起来.

结论:

参与RPE,BRB和免疫反应的蛋白质在JIA-U患者的眼中被发现在不同水平.
这些撕裂生物标志物可能提供一种非侵入性方法来检测JIA患者的脑膜炎.
这些已识别的标志物有可能用于早期诊断和监测JIA相关的脑膜炎.

关键词:

孩子孩子孩子孩子 JIA JIA 是一家国际投资公司.蛋白质基质的保护性撕裂的眼,撕裂的眼.紫外线炎 (uvéitis) 是一种

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