一种蛋白酶抑制剂Camostat Mesilate对患有刺激性肠综合征的患者肠道微生物功能的影响:一项随机的安慰剂对照试验研究
Motoyori Kanazawa1, Kentaro Miyamoto2, Michiko Kano1
1Department of Behavioral Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan.
Digestion
|November 25, 2024
概括
作为一种蛋白酶抑制剂的Camostat mesilate (CM) 在刺激性肠综合征 (IBS) 患者中改变了肠道细菌的功能,通过增加血清蛋白酶和FKBP型PPIase活性. 这表明在IBS治疗中准肠道微生物功能的潜力.
科学领域:
- 胃肠病学 胃肠病学
- 微生物组研究的研究.
- 药理学 药理学是指药理学的学科.
背景情况:
- 刺激性肠综合征 (IBS) 与便蛋白酶活性增加有关,可能导致内脏过敏.
- 血清蛋白酶影响FKBP类型的peptidylprolyl cis-trans isomerase (PPIase) 活性,影响免疫和葡萄皮质皮质体受体功能.
- 研究蛋白酶抑制剂,如卡莫斯塔特甲基酸 (CM),可能会揭示IBS的治疗点.
研究的目的:
- 为了确定卡莫斯塔特甲基酸盐 (CM) 是否改变了与IBS患者的FKBP型PPIases相关的便细菌功能.
- 评估CM对IBS症状,结肠运动功能和疼痛值的影响.
- 探索CM处理后便微生物组组成和功能的变化.
主要方法:
- 一个随机的,安慰剂对照试验,涉及32名IBS患者,接受CM或安慰剂治疗14天.
- 评估足够的缓解 (AR),IBS症状严重程度尺度 (IBS-SSS),结肠运动和治疗前后的疼痛值.
- 用PICRUSt和KEGG分析便16SrRNA基因测序,用于微生物组合和功能推断.
主要成果:
- 与安慰剂相比,CM显著增加了链球菌的丰度和血清蛋白酶和FKBP型PPIases (FkpA,FklB,SlyD) 的功能潜力.
- 在充分缓解 (AR) 方面,CM没有表现出对安慰剂的优越性.
- 在CM治疗后,结肠运动反应显示了部分变化.
结论:
- 在IBS患者中,Camostat mesilate调节了便微生物组合和与FKBP型PPIase活性相关的功能潜力.
- 蛋白酶抑制剂可能通过修改肠道微生物功能来代表治疗策略.
- 这些发现表明,在IBS的病理生理学中向蛋白酶活性可能发挥作用,可能会影响免疫或压力反应.
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