通过使用果对抗抗甲素耐药黄金葡萄球菌的新型自然和经济方法
Sherief M Abdel-Raheem1, Eman M Abouelhassan2, Mostafa Mandour3
1Department of Public Health, College of Veterinary Medicine, King Faisal University, P.O. Box 400, Al-Hofuf, Al-Ahsa, 31982, Saudi Arabia; Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Assiut University, Assiut, 71526, Egypt.
Microbial pathogenesis
|November 25, 2024
概括
果 (ACV) 在乳制品动物中表现出强大的抗菌作用,对多药耐药的甲素耐药黄金葡萄球菌 (MRSA) 产生强烈的抗菌作用. 这种天然替代品可以对抗抗生素耐药性,并降低乳腺炎治疗成本.
科学领域:
- 兽医微生物学 兽医微生物学
- 抗微生物耐药性 抗微生物耐药性
- 食品安全 食品安全
背景情况:
- 甲素耐药黄金葡萄球菌 (MRSA) 由于传染性乳腺炎,导致奶牛养殖业的重大经济损失.
- 多药耐药 (MDR) MRSA的增加需要新的抗菌策略,以减轻其传播和对动物和人类健康的影响.
- 埃及的乳腺炎治疗费用在2016-2021年期间估计为8200万EGP,这突显了经济负担.
研究的目的:
- 为了评估果 (ACV) 对来自乳制品动物的多药耐药MRSA分离物的抗菌功效.
- 为了对这些MRSA分离物中存在的抗微生物耐药性基因进行分析.
- 探索天然替代方法来对抗MRSA并减少与乳腺炎相关的经济损失.
主要方法:
- 从乳制品动物中分离和识别金黄色葡萄球菌,随后确认MRSA.
- 针对各种抗生素的MRSA分离物的抗菌敏感性测试和耐药基因 (mecA, blaZ, tetM, ermB) 的分析.
- 在体外评估ACV的抗菌活性,使用阿加井扩散技术,并确定最小抑制度 (MIC) 和最小杀菌度 (MBC).
主要成果:
- 在22个S. aureus分离物中,有19个 (86.4%) 确诊为MRSA,主要来自奶牛 (59.1%).
- 对克林达米辛 (94.7%),安培 (84.2%) 和多西环素 (84.2%) 观察到高耐药率. 范科米辛,西普罗撒辛和莱沃撒辛显示出对MRSA治疗的潜力.
- 在实验室中,ACV对MRSA表现出显著的抗菌活性,抑制区为20-40毫米,MIC值为2-4μg/ml.
结论:
- 果显示出对抗多药耐药MRSA有前途的抗菌特性,表明其作为天然替代治疗的潜力.
- 该研究在MRSA分离物中确定了关键的抗微生物耐药性基因,有助于了解耐药性机制.
- 牛奶可以帮助减少牛奶农场中抗生素耐药细菌的流行,提高农场效率,降低与乳腺炎相关的经济损失.
更多相关视频
相关概念视频
01:28Biological Methods for Microbial Control
2
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
2
01:29Methods for Controlling Microbial Growth
3
Microbial growth control refers to various methods employed to inhibit, reduce, or eliminate microorganisms to ensure safety and hygiene across different settings. These methods are categorized based on the target environment and the level of microbial control required.Biocides are versatile agents designed to control microorganisms by either inhibiting their growth or outright killing them. These agents work through various physical, chemical, mechanical, or biological mechanisms. The...
3
01:28Antimicrobial Effectiveness
2
The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
2
01:18Surface Membrane Barriers
1.0K
The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
1.0K
01:27Chemical Agents for Microbial Control
2
Chemicals play important roles in controlling microbial growth by targeting microbial structures and functions as sanitizers, antiseptics, disinfectants, and sterilants.Alcohols are commonly used sanitizers, effectively disrupting lipid membranes, which compromises cell integrity. They are also used as antiseptics and disinfectants due to their rapid action and versatility.Phenols and their derivatives phenolics , known for denaturing proteins and disrupting cell membranes, are particularly...
2
01:30Methods of Sterilization II: Chemical Methods
6.2K
In healthcare, the chemical method of sterilization uses chemical sterilants to treat surgical instruments and medical supplies to help prevent the transmission of infectious pathogens to patients. Due to heat sensitivity, most medical supplies and equipment should not be exposed to high temperatures. These parts include rubber, plastic, glass, and other similar elements.
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
6.2K