相关实验视频

Updated: Jun 6, 2025

Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling
11:55

Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling

Published on: May 29, 2011

15.0K

为单分子光漂白研究的膜蛋白进行囊特异性标记的路线图

Melanie Ernst1, Robyn Mahoney-Kruszka1, Nathan B Zelt1

  • 1Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO, USA.

Methods (San Diego, Calif.)
|November 25, 2024
PubMed
概括

选囊蛋白位点进行膜蛋白标记对于准确的单分子光漂白研究至关重要. 严格的测试显示,只有大约30%的合理选择的地点有效量化蛋白质寡合化.

关键词:
Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5 Cy5半氨酸 (cysteine) 是一种氨酸.埃尔曼·埃尔曼 (Ellman Ellman) 是一个马莱米胺是什么意思?马莱米胺是什么意思膜蛋白是一种膜蛋白质.摄影漂白是一种照片漂白.

更多相关视频

Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET10:59

Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET

Published on: August 17, 2022

3.1K
Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes10:43

Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes

Published on: July 19, 2022

2.2K

相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K