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相关概念视频

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Hybridoma Technology

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Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
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Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies
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抗体的克隆变异分析 工程图书馆

Ahmed S Fahad1,2, Matías F Gutiérrez-Gonzalez1,2, Bharat Madan1

  • 1The Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology, and Harvard University, Cambridge, Massachusetts 02139, USA.

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这项研究引入了一种生物信息学协议,用于在体外抗体进化过程中跟踪合成抗体变体. 这种方法允许在抗体显示库中精确分析单个突变函数,以改进抗体工程.

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科学领域:

  • 生物技术是生物技术.
  • 免疫学 免疫学 免疫学
  • 生物信息学是一种生物信息学.

背景情况:

  • 在体外抗体进化通过创造各种变体来增强单克隆抗体.
  • 抗体显示屏选这些变体以改善功能.
  • 在查轮中跟踪这些变异对于优化至关重要.

研究的目的:

  • 描述一种用于追踪合成抗体变体的生物信息协议.
  • 为了使单个突变函数的高通量解释.
  • 为了促进抗体工程和针对目标抗原的优化.

主要方法:

  • 开发一个生物信息学协议.
  • 使用高通量测序来追踪变体.
  • 在查轮中分析克隆抗体变异数据.

主要成果:

  • 对抗体变异函数的高通量高效解释.
  • 对个别突变效应的精确分析.
  • 能够发现改善的抗体功能.

结论:

  • 该协议为抗体工程和优化提供了一个强大的工具.
  • 它允许详细了解抗体库中的突变影响.
  • 通过系统分析,方便发现优质单克隆抗体.