增强蛋白产量:在Fusarium venenatum中代谢调节酸合成酶
Ruru Hong1,2,3, Sheng Tong2,3, Mengdan Chai2,3
1College of Bioengineering, Tianjin University of Science and Technology, Tianjin 300457, China.
Journal of agricultural and food chemistry
|November 25, 2024
概括
抑制Fusarium venenatum中素合成可以通过减少碳损失来提高真菌蛋白质的产生. 进一步的基因改造可以提高微生物蛋白的产量和转化效率.
科学领域:
- 生物技术是生物技术.
- 微生物发酵 微生物发酵
- 分子生物学分子生物学
背景情况:
- Fusarium venenatum被广泛用于微生物蛋白质的生产.
- 高食纤维含量导致在发酵过程中显著的碳损失.
- 丁生物合成的抑制是增加蛋白产量的潜在策略.
研究的目的:
- 为了确定参与F.venenatum.chitin合成的关键基因.
- 为了评估基因合成酶基因淘汰对发酵效率和蛋白质产量的影响.
- 探索进一步的基因改造,以优化真菌蛋白质的生产.
主要方法:
- 转录和生物信息学分析以识别基因.
- 基因淘汰实验用于创建突变菌株 (ΔFvChs3).
- 发酵试验以评估碳转化,蛋白质转化和乙醇生产.
主要成果:
- 鉴定出FvChs3基因对于胆合成至关重要.
- 淘汰FvChs3降低了26%的基含量,降低了47%的乙醇生产,但增加了16%和36%的碳和蛋白质转化.
- 在 ΔFvChs3 菌株中进一步淘汰一个 pyruvate decarboxylase 基因,碳和蛋白质的转化率提高到 29% 和 40%.
结论:
- 准基生物合成是提高F. venenatum蛋白质生产的有效策略.
- 对F. venenatum的基因工程可以显著提高碳和蛋白质转化效率.
- 这项研究为通过代谢工程优化微生物蛋白质生产提供了基础.
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