TBP激活DCBLD1转录以促进宫癌细胞周期进展
Zhigang Shen1, Mei Li1, He Zhu2
1Department of Pharmacy, Jilin Cancer Hospital, NO. 1066, Jinhu Road, High-tech District, Changchun, Jilin, 130012, P.R. China.
Functional & integrative genomics
|November 25, 2024
概括
塔塔盒结合蛋白 (TBP) 激活了迪斯科伊丁,CUB和LCCL域含有1 (DCBLD1) 的表达,促进了宫癌 (CC) 的进展. 抑制TBP或DCBLD1可能为CC提供新的治疗策略.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 癌症研究 癌症研究
背景情况:
- 迪斯科伊丁,CUB和LCCL域含有 (DCBLD) 蛋白与癌症预后不佳有关.
- 宫癌 (CC) 仍然是一个重要的全球健康挑战,需要新的治疗目标.
研究的目的:
- 研究DCBLD1在宫癌发展中的作用和机制.
- 确定DCBLD1在CC中的表达所涉及的调节因素.
主要方法:
- 生物信息分析用于识别CC中失调的基因.
- 免疫组织化学和RT-qPCR用于评估DCBLD1的表达.
- 细胞测试 (敲击,过度表达) 来评估功能影响.
- 染色体免疫沉和光酶记者测定证实转录因子结合.
- 在体内验证的异种移植小鼠模型.
主要成果:
- 在CC组织和细胞中,DCBLD1的表达显著上调.
- 在DCBLD1的淘汰下,抑制了CC细胞的增殖,入侵和迁移,同时促进了细胞亡和G1细胞循环停止.
- 鉴定出 TATA 盒结合蛋白 (TBP) 是 DCBLD1.1. 的转录激活剂.
- 结核蛋白倒置模仿了DCBLD1的倒置效应,但由于DCBLD1的过度表达,这些效应是可逆的.
- 在体内研究证实,TBP敲击抑制了瘤生长,DCBLD1过度表达恢复了瘤生长.
结论:
- 通过TBP介导的DCBLD1激活对于宫癌的进展和细胞周期调节至关重要.
- TBP和DCBLD1代表了宫癌治疗的潜在治疗点.
相关概念视频
Negative Regulator Molecules
35.2K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
35.2K
Inhibition of Cdk Activity
4.7K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.7K
DNA Damage can Stall the Cell Cycle
9.0K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.0K
Molecular Factors Affecting Cell Division
3.0K
Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
3.0K
Mitogens and the Cell Cycle
6.4K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.4K
Positive Regulator Molecules
5.4K
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
5.4K


