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EIF4A3诱导的hsa_circ_0127071通过JAK2/STAT5信号通路促进人类球间细胞衰老

Ying Chen¹, Xinwang Zhu¹, Da Sun¹

¹Department of Nephrology, The First Hospital of China Medical University, No.155 Nanjing Bei Street, Shenyang, Liaoning, China.

Scientific reports

|November 25, 2024

概括

循环RNA hsa_circ_0127071通过激活EIF4A3 / JAK2 / STAT5通路来加速脏衰老. 针对这种机制为抗衰老疗法和治疗脏疾病提供了新的策略.

科学领域:

老年学是一门学科.
分子生物学分子生物学
腎臟病學 (nephrology) 是一種醫學.

背景情况:

循环RNAs (circRNAs) 越来越多地被认为是它们在细胞过程中的作用,包括衰老.
了解细胞衰老中的circRNA机制对于确定新的抗衰老治疗点至关重要.

研究的目的:

研究hsa_circ_0127071在衰老中的作用和调节机制.
探索 hsa_circ_0127071 作为治疗年龄相关脏疾病的治疗点的潜力.

主要方法:

高通量测序以识别年轻与老年脏组织中的差异性circRNA表达.
定量实时PCR (qRT-PCR) 用于验证hsa_circ_0127071的表达式.
细胞衰老试验 (SA-β-Gal,马森三色染色).
为了阐明分子相互作用,RNA免疫沉 (RIP),RNA拉下测试和西部涂抹 (Western Blot, WB).

主要成果:

hsa_circ_0127071表达与年轻组织相比,在老年脏组织中显著增加.
沉默EIF4A3,JAK2 / STAT5通路的组成部分,延迟细胞衰老.
红色素 (EPO) 激活了JAK2/STAT5通路,增加了人类质介质细胞 (HGMC) 的衰老.
洛萨坦 (LOS) 治疗降低了JAK2/STAT5活性,并延迟了HGMC衰老.

结论:

hsa_circ_0127071通过EIF4A3/JAK2/STAT5信号轴促进衰老.
这种circRNA代表了治疗衰老和相关疾病的新治疗标.

关键词:

人类的球介质细胞是人类的球介质细胞.监管机制监管机制脏衰老脏衰老信号通道的信号通道.

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