相关实验视频

Updated: Jun 6, 2025

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

Published on: February 3, 2020

基因组范围的识别,表征和表达分析关键的基因家族在RNA沉默在百叶草沉默

Siyu Liu¹, Xiong Lei², Wenlong Gou³

¹College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu , Sichuan, 611130, China.

|November 26, 2024

概括

识别和分析了百叶草RNA沉默基因 (AGO,DCL,RDR) 在应激反应中的作用. 大多数基因在各种非生物压力下升调,这表明它们在草草弹性中的重要性.

科学领域:

植物分子生物学植物分子生物学
基因组学就是基因组学.
生物信息学是一种生物信息学.

背景情况:

涉及阿尔戈诺特 (AGO),迪克尔样 (DCL) 和依赖RNA的RNA聚合酶 (RDR) 蛋白质的RNA沉默通路对于植物发育和应激反应至关重要.
百叶草 (Eremochloa ophiuroides),一个重要的草草,具有有限的特征RNA沉默基因及其与压力相关的功能.

研究的目的:

为了进行全基因组识别和全面分析AGO,DCL和RDR基因家族.
研究这些基因在各种非生物压力下的进化关系和表达模式.

主要方法:

全基因组分析以识别和表征百叶草中的AGO,DCL和RDR基因成员.
进行了遗传学分析,基因重复,合成分析,动机发现,基因结构和cis作用元素分析.
用RT-qPCR检查不同组织的基因表达,以及在寒冷,盐,干旱,和除草剂压力下.

主要成果:

识别了20个AGO,6个DCL和10个RDR成员,并详细描述了它们的特征.
遗传学分析揭示了进化关系,得到3D蛋白质结构预测的支持.
大多数已识别的基因在应对多种非生物性压力时显示上调,这表明它们在耐压力方面具有特殊的作用.

结论:

这项研究提出了一项基本的全基因组基因组分析,对百叶草AGO,DCL和RDR基因家族进行了分析.
这些发现提供了关于这些基因在压力下表达模式的见解,这对于理解草草中的RNA沉默至关重要.
进一步的研究和针对这些基因的分子育种可以增强百叶草的抗压能力.

关键词:

在此之前,AGOGO.在 DCL DCL DCL 中.这就是为什么RDR是RDR.无生物性压力导致的压力.一百足草是一种草.基因家族基因家族

更多相关视频

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

Published on: July 11, 2020

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Published on: December 22, 2017

相关概念视频

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...