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单细胞RNA-Seq和组织学分析揭示了唾液腺发育过程中的动态Lrig1表达

Shumin Liu¹, Yuanyuan Li¹, Delan Huang¹

¹State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, China.

Journal of cellular physiology

|November 26, 2024

概括

富含白的重复和免疫球蛋白类域1 (LRIG1) 对于唾液腺发育至关重要. 这项研究表明LRIG1在原生细胞中的表达及其在调节唾液腺生长和成熟中的作用.

科学领域:

发展生物学发展生物学
干细胞生物学干细胞生物学
分子生物学分子生物学

背景情况:

唾液腺 (SG) 的发育涉及信号通路的复杂的时空调节.
干细胞/祖细胞及其血统是SG形态发生和成熟的关键.
富含白的重复和免疫球蛋白类域1 (LRIG1) 调节干细胞的增殖和静止.

研究的目的:

研究Lrig1在发育和成熟的小鼠下腺 (SMG) 中的表达模式和功能.

主要方法:

收集不同发育阶段的小鼠SMG.
使用qRT-PCR,免疫光 (IF) 和RNAscope染色检查了Lrig1表达.
利用单细胞RNA测序 (scRNA-Seq),转录因子 (TF) 预测,基因本体学 (GO) 分析,以及体外HSG细胞系实验.

主要成果:

Lrig1在胚胎和新生儿SMG上皮和介质细胞中广泛分布,局部化到成熟的上皮.
LRIG1在和基底细胞中高度缩在SMG原始标记物中.
实验室研究表明LRIG1影响人类唾液腺 (HSG) 细胞的增殖和迁移.

结论:

LRIG1在唾液腺发育中起着至关重要的作用,特别是在原生细胞群中.
了解LRIG1的功能提供了对SG形态发生和产后成熟的见解.
对LRIG1上游监管TF进行进一步的研究是有必要的.

关键词:

一个LRIG1的LRIG1 恒常状态 (homeostasis) 是一种平衡状态.唾液腺的发展唾液腺的发展在 scRNA-Seqq 中.干细胞是干细胞的组成部分.

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