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环-ECH1可能与miR-708-5p竞争,以调节支气管肺形症中的Ntrk2
Hanrong Cheng1, Dongcai Li2, Yuming Tang1
1Institute of Respiratory Diseases, Department of Sleep Medicine, Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, The First Affiliated Hospital of Southern University of Science and Technology, Shenzhen, China.
Journal of cellular biochemistry
|November 26, 2024
概括
循环RNAcirc-ECH1通过调节miR-708-5p和Ntrk2.2来预防支气管肺功能障碍 (BPD). 在BPD模型中,抑制circ-ECH1提高了细胞活力,并减少了细胞亡和纤维化.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 支气管肺功能障碍 (BPD) 是一种慢性肺部疾病,影响婴儿,影响生活质量.
- 循环RNAs (circRNAs) 在BPD病变发生过程中发挥作用,但circ-ECH1的功能仍然未被探索.
研究的目的:
- 研究circ-ECH1在支气管肺功能障碍症中的作用和潜在机制.
主要方法:
- 一个体外BPD模型使用高氧处理的L2细胞.
- 检测包括CCK-8,流细胞计,ROS检测,FISH,qRT-PCR,西部斑点和道染色.
- 双 luciferase 试验证实了 circ-ECH1 和 miR-708-5p 之间的相互作用.
主要成果:
- 过氧减少了细胞活力,增加了细胞亡和ROS,并降低了circ-ECH1的表达.
- Circ-ECH1过度表达增强了细胞活力,减少了细胞亡和ROS,并缓解了肺纤维化.
- 通过海绵化miR-708-5p,circ-ECH1调节了Ntrk2的表达,miR-708-5p模仿逆转circ-ECH1的影响.
结论:
- 通过调节circ-ECH1/miR-708-5p/Ntrk2轴,Circ-ECH1在BPD中起着保护因素的作用.
- Circ-ECH1代表了支气管肺功能障碍的潜在治疗标.
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