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人工修饰的NK细胞为癌症的基础的协同免疫基因光动力学疗法

Jiale Wu^1,2, Kaihong Shi^1,2, Wei Chao^1,2

¹Department of Pharmacy, Xuzhou Medical University, Xuzhou, People's Republic of China.

International journal of nanomedicine

|November 26, 2024

概括

这项研究介绍了一种智能自然杀手 (NK) 细胞药物递送系统 (DDS),该系统结合了免疫疗法,基因疗法和光动力疗法,用于增强癌症治疗. 新的DDS显示了固体瘤治疗的巨大潜力.

科学领域:

生物医学工程生物医学工程
纳米技术纳米技术
癌症治疗癌症治疗

背景情况:

自然杀手 (NK) 细胞具有强大的抗癌特性,但受到瘤微环境 (TME) 的阻碍.
由于免疫抑制TME机制,现有的免疫疗法在固体瘤中面临挑战.
需要一种新的药物递送系统 (DDS) 来克服TME介导的NK细胞疗法的抑制.

研究的目的:

设计和评估具有光响应性和TME响应性属性的智能NK细胞DDS.
为了提高NK细胞基癌症免疫疗法的疗效.
结合NK细胞免疫疗法,siRNA基因疗法和光动力疗法,以获得协同作用的抗癌效应.

主要方法:

开发了一种DDS (SP-NG@NKpH),使用用低pH插入 (pHLIP) 修改的NK细胞作为载体.
封装的siRNA和一个光敏感剂在一个减少反应的纳米凝 (NG) 中作为货物.
利用光激活和生物还原反应,在癌细胞内准确释放载荷.

主要成果:

该SP-NG@NKpH系统证明了通过pHLip通过癌细胞的向传递和定.
近红外激光照射触发了ROS生成和药物快速释放.
DDS在体外和体内表现出显著的瘤向特异性和增强的细胞毒性.

结论:

结合NK细胞免疫疗法,siRNA基因疗法和光动力学疗法的综合治疗方法产生了强大的抗癌效应.
这种多功能NK细胞DDS显示出对固体瘤治疗的有前途的临床应用前景.

关键词:

能够响应TME的设备.这是一种抗癌药物.细胞免疫疗法细胞免疫疗法纳米凝是一种纳米材料.自然杀手细胞是自然杀手细胞.照片响应式的照片响应式.

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