使用反应表面方法优化彩虹鱼内脏对氨基酸释放的自解
Haizea Domínguez1, Bruno Iñarra1, Jalel Labidi2
1Food Research, AZTI Foundation, Basque Research and Technology Alliance (BRTA), Derio, Bizkaia, 48160, Spain.
Open research Europe
|November 26, 2024
概括
彩虹鱼内脏通过自身溶解,使用内源性酶的价值化,产生了丰富的自由氨基酸 (FAA) 水解物. 最佳条件是pH8,40°C和6.85%的含水量,实现全球FAA产量略高于料.
科学领域:
- 生物技术是生物技术.
- 水产养殖是水产养殖的一种方式.
- 生物化学 生化学
背景情况:
- 彩虹鱼内脏在欧洲是一个重要的废物流,由于环境影响和加工挑战,需要回收利用.
- 鱼蛋白水解剂提供了一个有利可图的替代品,以传统的鱼粉生产从内脏.
- 使用内源性酶进行自解,成为从鱼内脏中产生水解盐的有希望的方法.
研究的目的:
- 通过响应表面方法来优化彩虹鱼内脏的自解条件.
- 为了确定自溶过程的动力参数.
- 为了比较自溶与化和酶化水解在水解盐生产中的有效性.
主要方法:
- 一个因数设计探讨了pH,温度和水含量对自溶的影响.
- 响应表面方法分析实验数据以确定最佳条件.
- 进行了一项动力学研究,以确定所需的自解时间.
主要成果:
- 确定最佳的自溶条件为pH8,40°C和6.85%的含水量,以最大限度地提高水解度和自由氨基酸 (FAA) 产量.
- pH 和添加的水含量是影响自溶过程的重要因素 (p < 0.05).
- 自溶过程需要7个小时才能有效,与料相比,每100克内脏的FAA总产量略高.
结论:
- 自溶是一种可行的方法,可以将彩虹鱼内脏提升为有价值的蛋白质水解剂.
- 内源性性蛋白酶有效地促进了富含氨基酸的自由解酸的产生.
- 自溶性过程为可持续利用鱼类加工副产品提供了一个有希望的途径.
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