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虹膜调节的lncRNAs及其潜在的调节功能在人类介质干细胞的chondrogenic分化中

Yijie Chen¹, Wenqi Sha¹, Yifan Zhang¹

¹Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Shaanxi, 710004, People's Republic of China.

Open medicine (Warsaw, Poland)

|November 26, 2024

概括

一种肌基因的虹膜素通过调节人类介质干细胞 (MSCs) 中的长非编码RNA (lncRNAs) 来影响体差异化. 这项研究确定了与骨关节炎 (OA) 治疗相关的素介导途径中的关键 lncRNA.

科学领域:

分子生物学分子生物学
细胞生物学细胞生物学
生物化学生物化学

背景情况:

骨关节炎 (OA) 涉及失调的冠状腺分化.
肌基因伊利辛显示了对OA的治疗潜力,但其机制尚不清楚.
长非编码RNAs (lncRNAs) 是冠状细胞分化的关键调节者.

研究的目的:

调查 lncRNAs 在调解虹膜激素诱导的混基分化中的作用.
在人类介质干细胞 (MSCs) 中识别虹膜调节的lncRNAs.

主要方法:

分析了用虹膜素治疗的人类MSC的全转录组测序数据.
预测的lncRNA目标和竞争性内源RNA (ceRNA) 相互作用.
对差异表达基因 (DEGs) 和 lncRNAs (DElncRNAs) 进行了功能丰富分析.

主要成果:

鉴定了许多由素诱导的差异表达的lncRNAs (DElncRNAs).
突出显示了与OA和冠状体发生相关的虹素诱导的顶部lncRNAs (例如,XIST,PAX8-AS1,CASC15,LINC01618,DLX6-AS1).
发现了与骨发育,细胞外基质 (ECM) 组织,细胞粘附和炎症的途径中丰富的DElncRNAs共同表达的DEGs.
建议的lncRNAs作为调节mRNAs的ceRNAs起作用,例如ROR2和SORBS1.1.

结论:

在人类的MSC中,在人类的MSC中,在人类的MSC中,在人类的MSC中,在人类的MSC中,在人类的MSC中,在人类的MSC中,在人类的MSC中,在人类的MSC中,在人类的MSC中,在人类的MSC中,在人类的MSC中.
需要进一步的研究,以充分描述关键的虹素调节lncRNAs在体生成中的特定作用.

关键词:

这就是为什么cRNAss.混凝土分化的差异化.共同表达是一种共同表达.虹膜是一种虹膜.在 lncRNAs 中.

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