在结直肠癌中HMGB1基因多态和表达的临床相关性和功能
Fang Wang1, Zhijun Huang2, Jianping Li3
1School of Pharmacy, Yancheng Teachers' University, Jiangsu, China.
Nucleosides, nucleotides & nucleic acids
|November 26, 2024
概括
一种特定的HMGB1基因变异 (rs34000982 Ins等位基因) 与降低结直肠癌 (CRC) 风险和进展有关. HMGB1基因表达也与免疫细胞透和抗CRC药物敏感性相关.
科学领域:
- 遗传学和分子生物学
- 在瘤学瘤学.
- 癌症研究 癌症研究
背景情况:
- 高水平的高流动性组盒1 (HMGB1) 蛋白质与结直肠癌 (CRC) 的发展和转移有关.
- 了解HMGB1的遗传变异和基因表达对于预测CRC风险和治疗反应至关重要.
研究的目的:
- 研究HMGB1基因中的功能性InDel多态性 (rs34000982) 与CRC易感性和瘤阶段之间的关联.
- 探索HMGB1基因表达与免疫细胞透和CRC患者药物敏感性的临床相关性.
主要方法:
- 600名CRC患者和600名健康对照者的基因定型使用聚合酶连锁反应-聚烯胺凝电泳用于rs34000982多态.
- 功能分析以确定rs34000982 Ins等位基因hsa-miR-944与HMGB1 3'未翻译区域之间的相互作用.
- 对HMGB1基因表达与免疫细胞透和抗CRC药物敏感性的相关性分析.
主要成果:
- rs34000982 Ins等位基因和Ins/Ins基因型显著与CRC的敏感性降低有关,特别是晚期 (III-IV).
- 携带Ins等位基因或Ins/Ins基因型与患上III-IV期CRC的风险较低相关.
- 功能性研究证实,Ins等位基因促进了hsa-miR-944与HMGB1mRNA的相互作用.
- HMGB1基因表达水平与免疫细胞透和对各种抗CRC药物的敏感性有关.
结论:
- 在中国人群中,HMGB1 rs34000982多态可能作为CRC易感性和进展的预测标记.
- HMGB1基因表达水平可能被用作预测抗CRC药物敏感性的生物标志物.
相关概念视频
01:33Pleiotropy
39.8K
Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...
39.8K
01:28Human Genetics
535
Human genetics provides a profound framework for understanding the interplay between genetic predispositions and human psychology. At the heart of this discipline lies the study of how genes influence physical traits, behaviors, and susceptibility to diseases. Each person carries a unique genetic code that subtly or significantly shapes their psychological and behavioral landscape.
The complex relationship between genetics and psychology is observable through common biological components such...
The complex relationship between genetics and psychology is observable through common biological components such...
535
02:23Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
02:30Histone Variants at the Centromere
4.3K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
4.3K
01:20Mismatch Repair
4.8K
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
4.8K