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相关概念视频

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
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相关实验视频

Updated: Jun 6, 2025

Using Expansion Microscopy to Physically Enlarge Whole-Mount Drosophila Embryos for Super-Resolution Imaging
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高通量膨胀显微镜可以实现可扩展的超分辨率成像.

John H Day1, Catherine M Della Santina1, Pema Maretich2

  • 1Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, United States.

eLife
|November 26, 2024
PubMed
概括

高通量膨胀显微镜 (HiExM) 允许在96孔板上同时对许多样本进行纳米尺度成像. 这种可扩展的超分辨率技术可以提高细胞变化的检测,比如药物对心脏细胞的影响.

关键词:
A549 细胞 A549 细胞这是心肌细胞 (cardiomyocytes).细胞生物学 细胞生物学细胞培养培养的细胞培养.人类 人类 人类 人类 人类 人类 人类

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科学领域:

  • 细胞生物学 细胞生物学
  • 显微镜技术 显微镜技术
  • 生物技术是生物技术.

背景情况:

  • 扩展显微镜 (ExM) 通过样本扩展提供纳米尺度成像.
  • 目前的ExM方法对于同时处理样本的吞吐量有限.

研究的目的:

  • 为扩展显微镜 (HiExM) 开发一个高通量平台.
  • 为了在标准的96井板中实现可扩展的超高分辨率成像.

主要方法:

  • 细胞被培养和处理在96井板用于HiExM.
  • HiExM实现了大约4.2倍的同位素扩张.
  • 结合HiExM与高通量共聚焦成像.

主要成果:

  • 在多个井口和板块中成功扩张.
  • HiExM检测到多克索鲁比对心肌细胞核DNA的剂量依赖性影响,未在未扩展样本中观察到.
  • 在药物治疗后,表现出更好的细胞表型检测.

结论:

  • HiExM显著提高了ExM的可扩展性和易用性.
  • 这种方法改善了用于药物查和生物研究的细微细胞变化的检测.
  • HiExM扩大了ExM用于可扩展的超高分辨率成像的应用.