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从CCD1072-SK纤维细胞生成iPSC的基因传递通过基于八甲胺的纳米颗粒

Hanife Sevgi Varlı^1,2, Meryem Akkurt Yıldırım¹, Kadriye Kızılbey³

¹Department of Molecular Biology and Genetics, Institute of Science and Technology, Yildiz Technical University, 34220 Istanbul, Türkiye.

Current issues in molecular biology

|November 26, 2024

概括

基于八甲基胺的固体脂质纳米颗粒 (OCTNPs) 有效地将人类细胞重新编程成诱导多能干细胞 (iPSC). 这种新的非病毒载体方法为干细胞的进步提供了更安全的替代方案.

科学领域:

生物技术是生物技术.
干细胞生物学干细胞生物学
纳米医学是一种纳米医学.

背景情况:

诱导多能干细胞 (iPSCs) 对再生医学具有前景.
对于iPSC重编程的传统病毒载体带来了安全问题.
开发非病毒传递系统对于有效和安全的iPSC产生至关重要.

研究的目的:

开发和描述基于八甲基胺的新型固体脂质纳米颗粒 (OCTNPs) 作为iPSC重编程的非病毒载体.
评估OCTNPs在将重编程因子传递到人类纤维细胞细胞中的效率.
确认产生的iPSC的多能性及其差异化潜力.

主要方法:

对OCTNPs的合成和表征 (大小,泽塔潜力).
使用OCTNPs对具有重编程因子的人类CCD1072-SK纤维细胞进行感染.
使用免疫光学,流细胞计,RT-qPCR和性酸酶试验对iPSCs进行表征.
通过使用5-azacytidine,将iPSC分化为心肌细胞样细胞.

主要成果:

成功合成了OCTNP,平均尺寸为178.9nm,正泽塔电位为22.8mV.
经贸国家和地区网络平台在提供重编程因素方面表现出高的传输效率 (82.0%).
生成的iPSC表达了关键的多能性标志物 (OCT4,SOX2,KLF4) 并显示了性酸酶活性.
iPSCs成功地分化为类似心肌细胞的细胞.

结论:

跨国OCTNP代表了iPSC重编程的新且有效的非病毒载体.
这种方法为病毒载体提供了更安全的替代方案,最大限度地降低了干细胞应用中的风险.
这些发现支持OCTNP在推进生物技术应用方面的潜力,包括组织工程和个性化医学.

关键词:

生物技术是生物技术.细胞培养培养的细胞培养.纳米粒子介导的基因传递重编程是重新编程.

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iPS Cell Differentiation

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

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Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...