相关实验视频
Updated: Jun 6, 2025

07:20
Assaying for Inorganic Polyphosphate in Bacteria
Published on: January 21, 2019
8.6K
通过Corynebacterium matruchotii和Streptococcus mutans进行细胞外酸盐调节和聚酸盐积累
Debarati Ghose1, Robert S Jones2
1Department of Biology, Texas A&M University, College Station, TX 77840, USA.
Dentistry journal
|November 26, 2024
概括
科里尼细菌 (Corynebacterium matruchotii) 能够有效地从其环境中去除正酸盐 (Pi),这与斯氏菌 (Streptococcus mutans. mutans.) 不同. 口腔细菌的这种Pi吸收可能对开发新的重矿化疗法至关重要.
科学领域:
- 口腔微生物学 口腔微生物学
- 生物化学 生物化学
- 牙科研究 牙科研究
背景情况:
- 微生物对 (Ca2+) 和正酸盐 (P) 的离子交换影响了面膜去矿化.
- 了解口腔微生物组在调节Ca2+和Pi中的作用,是有效矿化疗法的关键.
研究的目的:
- 研究口腔细菌,特别是*Corynebacterium matruchotii*和*Streptococcus mutans*的能力,以调节Ca2+和P水平.
- 评估这些细菌在改变矿物溶解度以改善口腔健康方面的潜力.
主要方法:
- 在细菌生长过程中,使用 Askorbic 酸试验量化了 Orthophosphate (Pi) 的摄取量.
- 介质中的 (Ca2+) 水平使用ICP-OES进行测量.
- 通过DAPI/polyP结合光检测出细菌多酸盐 (polyP) 的积累.
主要成果:
- *Corynebacterium matruchotii*显示了显著的Pi积累 (3.84 mmol/L) 和多P的形成.
- *菌突变菌*没有显著的Pi吸收或聚P积累.
- 两种细菌菌株都没有导致中等Ca2+水平显著降低.
结论:
- *Corynebacterium matruchotii*具有相当大的Pi吸收能力,与聚P合成有关.
- 这些发现表明,特定的口腔细菌对Pi调节是口腔健康的关键因素.
- 未来用于预防技术的体外测定应该考虑将具有显著Pi吸收能力的口腔细菌纳入.
相关概念视频
Cell Inclusions
2
Prokaryotic cells possess a variety of inclusions that play crucial roles in nutrient storage, metabolic processes, and environmental adaptation. These structures enable bacteria to thrive under fluctuating environmental conditions by storing essential resources and optimizing their metabolic efficiency.Carbon Storage: Poly-β-Hydroxybutyric Acid and Glycogen GranulesBacteria frequently store excess carbon in specialized granules. Poly-β-hydroxybutyric acid (PHB) granules are lipid...
2
Phosphorylation
50.0K
The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
50.0K
Bacterial Phylum Planctomycetes
Planctomycetes are a group of morphologically distinct bacteria predominantly classified into two orders: Planctomycetales and Brocadiales. These gram-negative bacteria exhibit unique features, including division by budding and the presence of stalks or appendages. Their cells are often found in rosette arrangements, and they are notable for possessing an S-layer in their cell envelope, which is relatively uncommon among bacteria. Additionally, Planctomycetes frequently exhibit intracellular...
Biofilms
1
Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
1
Bacterial Phylum Actinobacteria
Coryneform bacteria are gram-positive, aerobic, nonmotile rods that exhibit irregular, club-shaped, or V-shaped arrangements. Their V-shape results from snapping division, where the inner cell wall layer forms the cross-wall, while the outer layer remains intact until it ruptures on one side, causing the daughter cells to bend away.The primary genera are Corynebacterium and Arthrobacter. Corynebacterium includes diverse species, ranging from saprophytes to pathogens like Corynebacterium...
Bacterial Phylum Tenericutes
The phylum Tenericutes, which includes the single class Mollicutes, comprises bacteria that lack cell walls. The term "Mollicutes" derives from the Latin word mollis, meaning "soft." These organisms are among the smallest known and are commonly referred to as mycoplasmas due to the prominence of the genus Mycoplasma, which includes well-known human pathogens. Despite their inability to stain gram-positively (a result of their lack of cell walls), mycoplasmas are phylogenetically related to the...

