相关实验视频

Updated: Jul 20, 2026

Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos

Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos

Published on: April 28, 2017

在Nilaparvata lugens中的三个NlCstF基因的识别和功能分析

Shengli Jing¹, Feifei Wang¹, Aobo Ren¹

¹College of Life Sciences, Xinyang Normal University, Xinyang 464000, China.

|November 26, 2024

概括

这项研究研究了米害虫Nilaparvata lugens.CstF基因的功能. 沉默这些必不可少的基因扰乱了发育和繁殖,表明它们对RNAi害虫控制的潜力.

科学领域:

分子生物学分子生物学
遗传学遗传学是一个
昆虫学昆虫学是一门学科.

背景情况:

分裂刺激因子 (CstF) 复合体对于真核生物中的3'端前mRNA处理至关重要.
在主要的水害虫Nilaparvata lugens中,CstF子单元 (CstF50,CstF64,CstF77) 的功能作用在很大程度上尚未被探索.

研究的目的:

为了识别和描述N. lugens的CstF基因 (NlCstF50,NlCstF64,NlCstF77).
为了研究这些基因的时空表达模式.
用RNA干扰 (RNAi) 阐明NlCstF基因在N. lugens发育和繁殖中的功能意义.

主要方法:

基因鉴定和基因序列表征.
使用定量PCR进行时空表达分析.
通过双链RNA (dsRNA) 注射来使NlCstF基因沉默的RNA干扰 (RNAi).
在RNAi后的发育,生存和繁殖的表型分析.

主要成果:

N. lugens CstF 基因编码了昆虫物种中保存的蛋白质.
NlCstF基因在所有发育阶段和组织中都表达,在卵子和丸中含量很高.
RNAi有效地降低了NlCstF基因表达 (68-90%).
敲除NlCstF基因导致显著的致死率 (高达95.4%),变缺陷,卵产量减少和化率降低.

结论:

NlCstF基因对于Nilaparvata lugens的正常发育和繁殖至关重要.
用RNAi向这些基因是控制这一重要的农业害虫的有希望的策略.

关键词:

在NlCstF基因.棕色的植物跳棕色的植物跳致命的表型致命的表型变中断破坏变中断生殖生殖的繁殖.

更多相关视频

Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors in a Burkholderia gladioli Symbiont of Lagria villosa Beetles

Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors in a Burkholderia gladioli Symbiont of Lagria villosa Beetles

Published on: August 12, 2021

Author Spotlight: Evaluation of Entomopathogenic Fungi in Wild Monochamus alternatus Populations for Biocontrol Applications in Forest Wood Borers

Author Spotlight: Evaluation of Entomopathogenic Fungi in Wild Monochamus alternatus Populations for Biocontrol Applications in Forest Wood Borers

Published on: September 29, 2023

相关概念视频

Nuclear Localization Signals and Import

Nuclear Localization Signals and Import

Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of 2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...

Comparing Copy Number Variations and SNPs

Comparing Copy Number Variations and SNPs

Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...