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基于miR319的SlTCP2/LANCEOLATE活性抑制是调节番茄果形状的必要条件

Airton Carvalho¹, Mateus H Vicente¹, Leticia F Ferigolo¹

¹Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ), Universidade de São Paulo, 13418-900 Piracicaba, São Paulo, Brazil.

The Plant journal : for cell and molecular biology

|November 26, 2024

概括

微RNA对SlTCP2/LA的调节对于番茄果形状至关重要. 这种调节的丧失会通过在发育过程中改变细胞分裂和auxin反应而导致长长的果实.

科学领域:

植物生物学植物生物学
发育生物学是发展生物学.
遗传学遗传学是一个

背景情况:

果实形态发生依赖于细胞分裂和扩张.
微RNAs (miRNAs) 在植物发育中起着至关重要的作用.
这种TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) LANCEOLATE (TCP2/LA) 基因参与了植物结构.

研究的目的:

调查 miR319 调节 SlTCP2/LA 在番茄果物形态学中的作用.
了解果实形状的决定背后的分子机制.

主要方法:

对西红中半占主导地位的La Mutant的分析.
对 SlTCP2/LA,OVATE 和 SlYUCCA4.4 的基因表达分析.
研究花芽中的auxin反应.
研究miRNA介导的基因调节.

主要成果:

失去miR319调节导致SlTCP2/LA表达过早,影响骨发育期间的细胞分裂.
突变者表现出延长的水果形状,减少卵子数量,并改变了auxin反应.
SlTCP2/LA抑制了OVATE活动和SlYUCCA4的表达,影响了水果形态和辅酶信号传递.

结论:

通过miR319调节SlTCP2/LA对于正常的西红果形状至关重要.
在水果发育过程中确定了OVATE,SlTCP2/LA和auxin通路之间的新型分子联系.
微RNA调节和auxin动态可能是Solanaceae中水果形状演化的关键驱动因素.

关键词:

兰索拉特兰索拉特这种植物是Solanum lycopersicum.果子开发果子开发妇产科妇产科 miR31919 在线观看

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